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Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems

Selber, Klaus; Collén, Anna LU ; Hyytia, Teppo; Penttila, Merja; Tjerneld, Folke LU and Kula, Maria-Regina (2002) In Bioseparation 10(4-5). p.229-236
Abstract
The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is an optimum in the partition coefficient with respect to detergent concentration and a maximum for the partition coefficient and the yield with respect to temperature. These results were confirmed for the detergent C12E5 to demonstrate that these optima are due to the nature of polyoxyethylenes. Cloud point extraction was found to be only slightly... (More)
The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is an optimum in the partition coefficient with respect to detergent concentration and a maximum for the partition coefficient and the yield with respect to temperature. These results were confirmed for the detergent C12E5 to demonstrate that these optima are due to the nature of polyoxyethylenes. Cloud point extraction was found to be only slightly affected by pH. In the case studied extraction of whole broth is favourable for a high yield and partition coefficient, since fusion protein adhering to the cells can be solubilized. However some loss of detergent which remains in the fungal biomass was observed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aqueous two-phase systems, detergent-based systems, protein extraction, protein purificaton, cloud-point extraction
in
Bioseparation
volume
10
issue
4-5
pages
229 - 236
publisher
Springer
external identifiers
  • scopus:0035553492
ISSN
1573-8272
DOI
10.1023/A:1016379111336
language
English
LU publication?
yes
id
a20e37a5-2b06-437b-b8bc-f246b260a4f7 (old id 124882)
date added to LUP
2007-07-06 15:05:33
date last changed
2017-07-02 04:29:31
@article{a20e37a5-2b06-437b-b8bc-f246b260a4f7,
  abstract     = {The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is an optimum in the partition coefficient with respect to detergent concentration and a maximum for the partition coefficient and the yield with respect to temperature. These results were confirmed for the detergent C12E5 to demonstrate that these optima are due to the nature of polyoxyethylenes. Cloud point extraction was found to be only slightly affected by pH. In the case studied extraction of whole broth is favourable for a high yield and partition coefficient, since fusion protein adhering to the cells can be solubilized. However some loss of detergent which remains in the fungal biomass was observed.},
  author       = {Selber, Klaus and Collén, Anna and Hyytia, Teppo and Penttila, Merja and Tjerneld, Folke and Kula, Maria-Regina},
  issn         = {1573-8272},
  keyword      = {aqueous two-phase systems,detergent-based systems,protein extraction,protein purificaton,cloud-point extraction},
  language     = {eng},
  number       = {4-5},
  pages        = {229--236},
  publisher    = {Springer},
  series       = {Bioseparation},
  title        = {Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems},
  url          = {http://dx.doi.org/10.1023/A:1016379111336},
  volume       = {10},
  year         = {2002},
}