Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems
(2002) In Bioseparation 10(4-5). p.229-236- Abstract
- The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is an optimum in the partition coefficient with respect to detergent concentration and a maximum for the partition coefficient and the yield with respect to temperature. These results were confirmed for the detergent C12E5 to demonstrate that these optima are due to the nature of polyoxyethylenes. Cloud point extraction was found to be only slightly... (More)
- The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is an optimum in the partition coefficient with respect to detergent concentration and a maximum for the partition coefficient and the yield with respect to temperature. These results were confirmed for the detergent C12E5 to demonstrate that these optima are due to the nature of polyoxyethylenes. Cloud point extraction was found to be only slightly affected by pH. In the case studied extraction of whole broth is favourable for a high yield and partition coefficient, since fusion protein adhering to the cells can be solubilized. However some loss of detergent which remains in the fungal biomass was observed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/124882
- author
- Selber, Klaus ; Collén, Anna LU ; Hyytia, Teppo ; Penttila, Merja ; Tjerneld, Folke LU and Kula, Maria-Regina
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- aqueous two-phase systems, detergent-based systems, protein extraction, protein purificaton, cloud-point extraction
- in
- Bioseparation
- volume
- 10
- issue
- 4-5
- pages
- 229 - 236
- publisher
- Springer
- external identifiers
-
- scopus:0035553492
- ISSN
- 1573-8272
- DOI
- 10.1023/A:1016379111336
- language
- English
- LU publication?
- yes
- id
- a20e37a5-2b06-437b-b8bc-f246b260a4f7 (old id 124882)
- date added to LUP
- 2016-04-01 17:02:43
- date last changed
- 2022-01-28 23:55:41
@article{a20e37a5-2b06-437b-b8bc-f246b260a4f7, abstract = {{The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is an optimum in the partition coefficient with respect to detergent concentration and a maximum for the partition coefficient and the yield with respect to temperature. These results were confirmed for the detergent C12E5 to demonstrate that these optima are due to the nature of polyoxyethylenes. Cloud point extraction was found to be only slightly affected by pH. In the case studied extraction of whole broth is favourable for a high yield and partition coefficient, since fusion protein adhering to the cells can be solubilized. However some loss of detergent which remains in the fungal biomass was observed.}}, author = {{Selber, Klaus and Collén, Anna and Hyytia, Teppo and Penttila, Merja and Tjerneld, Folke and Kula, Maria-Regina}}, issn = {{1573-8272}}, keywords = {{aqueous two-phase systems; detergent-based systems; protein extraction; protein purificaton; cloud-point extraction}}, language = {{eng}}, number = {{4-5}}, pages = {{229--236}}, publisher = {{Springer}}, series = {{Bioseparation}}, title = {{Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems}}, url = {{http://dx.doi.org/10.1023/A:1016379111336}}, doi = {{10.1023/A:1016379111336}}, volume = {{10}}, year = {{2002}}, }