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Degradation of Mannan I and II Crystals by Fungal endo-β-1,4-Mannanases and a β-1,4-Mannosidase Studied with Transmission Electron Microscopy

Hägglund, Per LU ; Sabini, Elisabetta; Boisset, Claire; Wilson, Keith; Chanzy, Henri and Stålbrand, Henrik LU (2001) In Biomacromolecules 2(3). p.694-699
Abstract
We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the... (More)
We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the digestion results give some further information about the ultrastructure of mannan II. In addition, insight is provided into the mode of the enzymatic attack on the crystals of mannan I and mannan II. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
2
issue
3
pages
694 - 699
publisher
The American Chemical Society
external identifiers
  • wos:000174256300012
  • scopus:0034831908
ISSN
1526-4602
DOI
10.1021/bm010006w
language
English
LU publication?
yes
id
d95c5852-096d-41b6-b4dd-70b06b25a1f5 (old id 125000)
date added to LUP
2007-07-05 14:52:08
date last changed
2018-01-07 06:23:44
@article{d95c5852-096d-41b6-b4dd-70b06b25a1f5,
  abstract     = {We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the digestion results give some further information about the ultrastructure of mannan II. In addition, insight is provided into the mode of the enzymatic attack on the crystals of mannan I and mannan II.},
  author       = {Hägglund, Per and Sabini, Elisabetta and Boisset, Claire and Wilson, Keith and Chanzy, Henri and Stålbrand, Henrik},
  issn         = {1526-4602},
  language     = {eng},
  number       = {3},
  pages        = {694--699},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Degradation of Mannan I and II Crystals by Fungal endo-β-1,4-Mannanases and a β-1,4-Mannosidase Studied with Transmission Electron Microscopy},
  url          = {http://dx.doi.org/10.1021/bm010006w},
  volume       = {2},
  year         = {2001},
}