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The C-terminus of dUTPase: observation on flexibility using NMR

Nord, Johan; Nyman, Per-Olof LU ; Larsson, Gunilla and Drakenberg, Torbjörn LU (2001) In FEBS Letters 492(3). p.228-232
Abstract
The dynamics of the C-terminus of the dUTPases from Escherichia coli and equine infectious anaemia virus (EIAV) were studied by 1H-15N nuclear magnetic resonance spectroscopy. The two enzymes differ with regard to flexibility in the backbone of the 15 most C-terminal amino acid residues, some of which are conserved and essential for enzymic activity. In the bacterial enzyme, the residues closest to the C-terminus are highly flexible and display a correlation time in the nanosecond time range. No similar high flexibility could be detected for the C-terminal part of EIAV dUTPase, indicating a different time range of flexibility.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dUTPase, Equine infectious anemia virus, Flexible C-terminus, Nuclear magnetic resonance, Nucleotide binding, Escherichia coli
in
FEBS Letters
volume
492
issue
3
pages
228 - 232
publisher
Wiley-Blackwell
external identifiers
  • scopus:0035896405
ISSN
1873-3468
DOI
10.1016/S0014-5793(01)02257-8
language
English
LU publication?
yes
id
9e09bd37-b249-46bc-ba6f-92ef2d4e36b9 (old id 125038)
date added to LUP
2007-07-09 08:30:04
date last changed
2018-05-29 10:01:19
@article{9e09bd37-b249-46bc-ba6f-92ef2d4e36b9,
  abstract     = {The dynamics of the C-terminus of the dUTPases from Escherichia coli and equine infectious anaemia virus (EIAV) were studied by 1H-15N nuclear magnetic resonance spectroscopy. The two enzymes differ with regard to flexibility in the backbone of the 15 most C-terminal amino acid residues, some of which are conserved and essential for enzymic activity. In the bacterial enzyme, the residues closest to the C-terminus are highly flexible and display a correlation time in the nanosecond time range. No similar high flexibility could be detected for the C-terminal part of EIAV dUTPase, indicating a different time range of flexibility.},
  author       = {Nord, Johan and Nyman, Per-Olof and Larsson, Gunilla and Drakenberg, Torbjörn},
  issn         = {1873-3468},
  keyword      = {dUTPase,Equine infectious anemia virus,Flexible C-terminus,Nuclear magnetic resonance,Nucleotide binding,Escherichia coli},
  language     = {eng},
  number       = {3},
  pages        = {228--232},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {The C-terminus of dUTPase: observation on flexibility using NMR},
  url          = {http://dx.doi.org/10.1016/S0014-5793(01)02257-8},
  volume       = {492},
  year         = {2001},
}