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Effect of feed supplementation with phytohaemagglutinin in combination with alpha-ketoglutarate on growth and nitrogen elimination pathways in rats with acute renal failure induced by nephrectomy

Filip, Rafal; Harrison, Adrian P. and Pierzynowski, Stefan LU (2008) In Archives of Medical Science 4(2). p.122-128
Abstract
Introduction: Phytohaemagglutinin (PHA) and alpha-ketoglutaric acid (AKG) in growing rats stimulate a change in the proportion of N excretion via urine and faeces, in favour of faecal excretion. The aim of the study was to investigate the effect of oral supplementation of PHA and AKG on pathways of nitrogen excretion and serum levels of urea in uraemic conditions induced by nephrectomy. Material and methods: Experiment 1 - 12 rats were assigned to one of two groups, control and PHA. Experiment 2 - PHA was administered to 36 male rats which were assigned to 4 groups: 1) uraemic control, 2) uraemic + AKG, 3) Shamoperated, 4) Sham-operated + AKG. AKG was administered via drinking water, while PHA was administered via a stomach tube. Results:... (More)
Introduction: Phytohaemagglutinin (PHA) and alpha-ketoglutaric acid (AKG) in growing rats stimulate a change in the proportion of N excretion via urine and faeces, in favour of faecal excretion. The aim of the study was to investigate the effect of oral supplementation of PHA and AKG on pathways of nitrogen excretion and serum levels of urea in uraemic conditions induced by nephrectomy. Material and methods: Experiment 1 - 12 rats were assigned to one of two groups, control and PHA. Experiment 2 - PHA was administered to 36 male rats which were assigned to 4 groups: 1) uraemic control, 2) uraemic + AKG, 3) Shamoperated, 4) Sham-operated + AKG. AKG was administered via drinking water, while PHA was administered via a stomach tube. Results: Lower daily weight gain (P<0.05), increase in small intestine and total GI tract weight (P<0.05) as well as significant reduction in N excretion in urine in the PHA group were observed (P<0.05). Significantly higher daily weight loss in the uraemic rats, compared to that of the sham-operated rats, was observed (P<0.05). A significant increase in N excretion in faeces was observed in the AKG group, compared to control within the sham-operated rats (P<0.05) and when compared to the uraemic rats (P<0.05). in both sham-operated and uraemic rats, AKG treatment led to a significant reduction in the urea levels (P<0.05). Conclusions: The change in the proportion of N excretion via urine and faeces caused by PHA due to increasing the rate of protein production in the intestinal wall, apparently favouring faecal excretion, can be enhanced by the oral administration of AKG. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nitrogen elimination, alpha-ketoglutaric acid, phytohaemagglutinin, intestine, Rat, ARF
in
Archives of Medical Science
volume
4
issue
2
pages
122 - 128
publisher
Termedia Publishing House
external identifiers
  • wos:000258602800004
ISSN
1734-1922
language
English
LU publication?
yes
id
54045a26-eb48-43b6-a24a-dd5f0d61c629 (old id 1252051)
alternative location
http://www.termedia.pl/magazine.php?magazine_id=19&article_id=10661&magazine_subpage=ABSTRACT
date added to LUP
2008-11-04 09:32:15
date last changed
2016-04-15 18:58:33
@article{54045a26-eb48-43b6-a24a-dd5f0d61c629,
  abstract     = {Introduction: Phytohaemagglutinin (PHA) and alpha-ketoglutaric acid (AKG) in growing rats stimulate a change in the proportion of N excretion via urine and faeces, in favour of faecal excretion. The aim of the study was to investigate the effect of oral supplementation of PHA and AKG on pathways of nitrogen excretion and serum levels of urea in uraemic conditions induced by nephrectomy. Material and methods: Experiment 1 - 12 rats were assigned to one of two groups, control and PHA. Experiment 2 - PHA was administered to 36 male rats which were assigned to 4 groups: 1) uraemic control, 2) uraemic + AKG, 3) Shamoperated, 4) Sham-operated + AKG. AKG was administered via drinking water, while PHA was administered via a stomach tube. Results: Lower daily weight gain (P&lt;0.05), increase in small intestine and total GI tract weight (P&lt;0.05) as well as significant reduction in N excretion in urine in the PHA group were observed (P&lt;0.05). Significantly higher daily weight loss in the uraemic rats, compared to that of the sham-operated rats, was observed (P&lt;0.05). A significant increase in N excretion in faeces was observed in the AKG group, compared to control within the sham-operated rats (P&lt;0.05) and when compared to the uraemic rats (P&lt;0.05). in both sham-operated and uraemic rats, AKG treatment led to a significant reduction in the urea levels (P&lt;0.05). Conclusions: The change in the proportion of N excretion via urine and faeces caused by PHA due to increasing the rate of protein production in the intestinal wall, apparently favouring faecal excretion, can be enhanced by the oral administration of AKG.},
  author       = {Filip, Rafal and Harrison, Adrian P. and Pierzynowski, Stefan},
  issn         = {1734-1922},
  keyword      = {nitrogen elimination,alpha-ketoglutaric acid,phytohaemagglutinin,intestine,Rat,ARF},
  language     = {eng},
  number       = {2},
  pages        = {122--128},
  publisher    = {Termedia Publishing House},
  series       = {Archives of Medical Science},
  title        = {Effect of feed supplementation with phytohaemagglutinin in combination with alpha-ketoglutarate on growth and nitrogen elimination pathways in rats with acute renal failure induced by nephrectomy},
  volume       = {4},
  year         = {2008},
}