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Fractionation of the thylakoid membranes from tobacco. A tentative isolation of ‘end membrane’ and purified ‘stroma lamellae’ membranes

Gadjieva, Rena LU ; Mamedov, Fikret LU and Albertsson, Per-Åke LU (1999) In Biochimica et Biophysica Acta - Bioenergetics 1411(1). p.92-100
Abstract
Thylakoids isolated from tobacco were fragmented by sonication and the vesicles so obtained were separated by partitioning in aqueous polymer two-phase systems. By this procedure, grana vesicles were separated from stroma exposed membrane vesicles. The latter vesicles could be further fractionated by countercurrent distribution, with dextran-polyethylene glycol phase systems, and divided into two main populations, tentatively named 'stroma lamellae' and 'end membrane'. Both these vesicle preparations have high chlorophyll a/b ratio, high photosystem (PS) I and low PS II content, suggesting their origin from stroma exposed regions of the thylakoid. The two vesicle populations have been compared with respect to biochemical composition and... (More)
Thylakoids isolated from tobacco were fragmented by sonication and the vesicles so obtained were separated by partitioning in aqueous polymer two-phase systems. By this procedure, grana vesicles were separated from stroma exposed membrane vesicles. The latter vesicles could be further fractionated by countercurrent distribution, with dextran-polyethylene glycol phase systems, and divided into two main populations, tentatively named 'stroma lamellae' and 'end membrane'. Both these vesicle preparations have high chlorophyll a/b ratio, high photosystem (PS) I and low PS II content, suggesting their origin from stroma exposed regions of the thylakoid. The two vesicle populations have been compared with respect to biochemical composition and photosynthetic activity. The 'end membrane' has a higher chlorophyll a/b ratio (5.7 vs. 4.7), higher P700 content (4.7 vs. 3.3 mmol/mol of chlorophyll). The 'end membrane' has the lowest PS II content, the ratio PS I/PS II being more than 10, as shown by EPR measurements. The PS II in both fractions is of the β-type. The decay of fluorescence is different for the two populations, the 'stroma lamellae' showing a very slow decay even in the presence of K3Fe(CN)6 as an acceptor. The two vesicle populations have very different surface properties: the end membranes prefer the upper phase much more than the stroma lamellae, a fact which was utilized for their separation. Arguments are presented which support the suggestion that the two vesicle populations originate from the grana end membranes and the stroma lamellae, respectively. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Thylakoid, End membrane, Stroma lamellae, Aqueous two-phase partitioning, Photosystem I, (Nicotiana tabacum)
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1411
issue
1
pages
92 - 100
publisher
Elsevier
external identifiers
  • scopus:0032910239
ISSN
0005-2728
DOI
10.1016/S0005-2728(99)00010-9
language
English
LU publication?
yes
id
ebb95b04-0da3-431a-811d-839838d890e7 (old id 125289)
date added to LUP
2007-07-05 09:46:43
date last changed
2017-01-01 07:25:41
@article{ebb95b04-0da3-431a-811d-839838d890e7,
  abstract     = {Thylakoids isolated from tobacco were fragmented by sonication and the vesicles so obtained were separated by partitioning in aqueous polymer two-phase systems. By this procedure, grana vesicles were separated from stroma exposed membrane vesicles. The latter vesicles could be further fractionated by countercurrent distribution, with dextran-polyethylene glycol phase systems, and divided into two main populations, tentatively named 'stroma lamellae' and 'end membrane'. Both these vesicle preparations have high chlorophyll a/b ratio, high photosystem (PS) I and low PS II content, suggesting their origin from stroma exposed regions of the thylakoid. The two vesicle populations have been compared with respect to biochemical composition and photosynthetic activity. The 'end membrane' has a higher chlorophyll a/b ratio (5.7 vs. 4.7), higher P700 content (4.7 vs. 3.3 mmol/mol of chlorophyll). The 'end membrane' has the lowest PS II content, the ratio PS I/PS II being more than 10, as shown by EPR measurements. The PS II in both fractions is of the β-type. The decay of fluorescence is different for the two populations, the 'stroma lamellae' showing a very slow decay even in the presence of K3Fe(CN)6 as an acceptor. The two vesicle populations have very different surface properties: the end membranes prefer the upper phase much more than the stroma lamellae, a fact which was utilized for their separation. Arguments are presented which support the suggestion that the two vesicle populations originate from the grana end membranes and the stroma lamellae, respectively.},
  author       = {Gadjieva, Rena and Mamedov, Fikret and Albertsson, Per-Åke},
  issn         = {0005-2728},
  keyword      = {Thylakoid,End membrane,Stroma lamellae,Aqueous two-phase partitioning,Photosystem I,(Nicotiana tabacum)},
  language     = {eng},
  number       = {1},
  pages        = {92--100},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Fractionation of the thylakoid membranes from tobacco. A tentative isolation of ‘end membrane’ and purified ‘stroma lamellae’ membranes},
  url          = {http://dx.doi.org/10.1016/S0005-2728(99)00010-9},
  volume       = {1411},
  year         = {1999},
}