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Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding

Silow, Maria; Tan, Yee-Joo; Fersht, Alan R and Oliveberg, Mikael LU (1999) In Biochemistry 38(40). p.13006-13012
Abstract
Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein concentrations, it is likely that the aggregates also form directly from the coil. This is in contrast to the behavior of larger multistate proteins where aggregation occurs in connection to "sticky" intermediates.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
38
issue
40
pages
13006 - 13012
publisher
The American Chemical Society
external identifiers
  • scopus:0032878322
ISSN
0006-2960
DOI
10.1021/bi9909997
language
English
LU publication?
yes
id
3efcff80-81a3-4f67-a06d-2176ca2646ef (old id 125407)
date added to LUP
2007-07-06 15:13:53
date last changed
2017-01-01 04:50:12
@article{3efcff80-81a3-4f67-a06d-2176ca2646ef,
  abstract     = {Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein concentrations, it is likely that the aggregates also form directly from the coil. This is in contrast to the behavior of larger multistate proteins where aggregation occurs in connection to "sticky" intermediates.},
  author       = {Silow, Maria and Tan, Yee-Joo and Fersht, Alan R and Oliveberg, Mikael},
  issn         = {0006-2960},
  language     = {eng},
  number       = {40},
  pages        = {13006--13012},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding},
  url          = {http://dx.doi.org/10.1021/bi9909997},
  volume       = {38},
  year         = {1999},
}