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Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography

Medve, József; Lee, Dora and Tjerneld, Folke LU (1998) In Journal of Chromatography A 808(1-2). p.153-165
Abstract
Trichoderma cellulases appear in several isoforms which makes their purification and analysis difficult. We used fast protein liquid chromatography (FPLC) to purify three major cellulases and to quantitate these enzymes in reconstituted mixtures during cellulose hydrolysis studies (in lack of specific substrates and because of the synergism between the enzymes such analysis is very difficult, if at all possible, with conventional activity measurements). For the analysis methods linear calibration was achieved from 10-15 pmol to 0.5-1 nmol (from 0.5-0.8 to 27-64 μg) for the different enzymes. Due to the high resolution chromatographic media used, our purification methods are simpler and quicker than the usual protocols for cellulase... (More)
Trichoderma cellulases appear in several isoforms which makes their purification and analysis difficult. We used fast protein liquid chromatography (FPLC) to purify three major cellulases and to quantitate these enzymes in reconstituted mixtures during cellulose hydrolysis studies (in lack of specific substrates and because of the synergism between the enzymes such analysis is very difficult, if at all possible, with conventional activity measurements). For the analysis methods linear calibration was achieved from 10-15 pmol to 0.5-1 nmol (from 0.5-0.8 to 27-64 μg) for the different enzymes. Due to the high resolution chromatographic media used, our purification methods are simpler and quicker than the usual protocols for cellulase purification. Several isoforms of cellobiohydrolase (CBH) I were purified. The isoforms had different isoelectric points (pI) but their catalytic and adsorption properties were similar. A remarkable feature of CBH I and endoglucanase (EG) II was that their electrophoretically pure preparations gave double peaks during ion-exchange chromatography in certain pH intervals where the two peaks (probably representing two conformations) were transformed into each other by changing pH. This behaviour of cellulases has never been reported before and further explains the difficulties in cellulase purification. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzymes, Cellulases, Cellobiohydrolases, Endoglucanases
in
Journal of Chromatography A
volume
808
issue
1-2
pages
153 - 165
publisher
Elsevier
external identifiers
  • scopus:0032577330
ISSN
0021-9673
DOI
10.1016/S0021-9673(98)00132-0
language
English
LU publication?
yes
id
c8d869cc-76d3-41b2-9e3a-2fe9068cd258 (old id 125578)
date added to LUP
2007-07-06 11:59:00
date last changed
2017-08-13 04:17:14
@article{c8d869cc-76d3-41b2-9e3a-2fe9068cd258,
  abstract     = {Trichoderma cellulases appear in several isoforms which makes their purification and analysis difficult. We used fast protein liquid chromatography (FPLC) to purify three major cellulases and to quantitate these enzymes in reconstituted mixtures during cellulose hydrolysis studies (in lack of specific substrates and because of the synergism between the enzymes such analysis is very difficult, if at all possible, with conventional activity measurements). For the analysis methods linear calibration was achieved from 10-15 pmol to 0.5-1 nmol (from 0.5-0.8 to 27-64 μg) for the different enzymes. Due to the high resolution chromatographic media used, our purification methods are simpler and quicker than the usual protocols for cellulase purification. Several isoforms of cellobiohydrolase (CBH) I were purified. The isoforms had different isoelectric points (pI) but their catalytic and adsorption properties were similar. A remarkable feature of CBH I and endoglucanase (EG) II was that their electrophoretically pure preparations gave double peaks during ion-exchange chromatography in certain pH intervals where the two peaks (probably representing two conformations) were transformed into each other by changing pH. This behaviour of cellulases has never been reported before and further explains the difficulties in cellulase purification.},
  author       = {Medve, József and Lee, Dora and Tjerneld, Folke},
  issn         = {0021-9673},
  keyword      = {Enzymes,Cellulases,Cellobiohydrolases,Endoglucanases},
  language     = {eng},
  number       = {1-2},
  pages        = {153--165},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography},
  url          = {http://dx.doi.org/10.1016/S0021-9673(98)00132-0},
  volume       = {808},
  year         = {1998},
}