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Up-regulation of endothelin receptor function and mRNA expression in airway smooth muscle cells following sephadex-induced airway inflammation.

Granström, Bengt LU ; Xu, Cang-Bao LU ; Nilsson, Elisabeth LU ; Bengtsson, Ursula Hultkvist and Edvinsson, Lars LU (2004) In Basic & Clinical Pharmacology & Toxicology2004-01-01+01:002014-01-01+01:00 95(1). p.43-48
Abstract
The hypothesis that up-regulation of bronchial constrictor endothelin receptors in airway smooth muscle cells may contribute to hyperreactivity during airway inflammation was tested in the present study by quantitative endothelin receptor mRNA analysis and functional responses in ring segments of rat trachea and bronchi. Real time reverse transcription polymerase chain reaction was used to quantify endothelin receptor expression in rat airway smooth muscle cells following Sephadex-induced inflammation. Compared with controls, Sephadex-induced airway inflammation caused a significant increase (3.9 times P<0.05) of endothelin receptor type B mRNA expression in bronchial smooth muscle cells, but not in tracheal smooth muscle cells.... (More)
The hypothesis that up-regulation of bronchial constrictor endothelin receptors in airway smooth muscle cells may contribute to hyperreactivity during airway inflammation was tested in the present study by quantitative endothelin receptor mRNA analysis and functional responses in ring segments of rat trachea and bronchi. Real time reverse transcription polymerase chain reaction was used to quantify endothelin receptor expression in rat airway smooth muscle cells following Sephadex-induced inflammation. Compared with controls, Sephadex-induced airway inflammation caused a significant increase (3.9 times P<0.05) of endothelin receptor type B mRNA expression in bronchial smooth muscle cells, but not in tracheal smooth muscle cells. Functional myograph studies of bronchial and tracheal ring segments without epithelium (mechanically denuded) revealed an increase of the maximum contractile effects of endothelin-1 (a dual agonist for both endothelin type A and B receptors) and sarafotoxin 6c (a selective agonist for endothelin B receptors) in bronchial smooth muscle cells in Sephadex-induced inflammation, but not in tracheal smooth muscle cells. The enhanced maximal responses of bronchial smooth muscle cells to endothelin-1 and sarafotoxin 6c in Sephadex-induced inflammation support our molecular findings and hence imply a role for endothelin B receptors in airway hyperreactivity during airway inflammation. (Less)
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Contribution to journal
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published
subject
in
Basic & Clinical Pharmacology & Toxicology2004-01-01+01:002014-01-01+01:00
volume
95
issue
1
pages
43 - 48
publisher
Wiley-Blackwell
external identifiers
  • pmid:15245576
  • wos:000222924000009
  • scopus:3342989772
ISSN
1742-7843
DOI
10.1111/j.1742-7843.2004.pto950109.x
language
English
LU publication?
yes
id
7010e60f-7c00-4784-b6ec-a28f28d08f16 (old id 125898)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=15245576&ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
date added to LUP
2007-06-29 09:02:29
date last changed
2017-08-27 03:50:31
@article{7010e60f-7c00-4784-b6ec-a28f28d08f16,
  abstract     = {The hypothesis that up-regulation of bronchial constrictor endothelin receptors in airway smooth muscle cells may contribute to hyperreactivity during airway inflammation was tested in the present study by quantitative endothelin receptor mRNA analysis and functional responses in ring segments of rat trachea and bronchi. Real time reverse transcription polymerase chain reaction was used to quantify endothelin receptor expression in rat airway smooth muscle cells following Sephadex-induced inflammation. Compared with controls, Sephadex-induced airway inflammation caused a significant increase (3.9 times P&lt;0.05) of endothelin receptor type B mRNA expression in bronchial smooth muscle cells, but not in tracheal smooth muscle cells. Functional myograph studies of bronchial and tracheal ring segments without epithelium (mechanically denuded) revealed an increase of the maximum contractile effects of endothelin-1 (a dual agonist for both endothelin type A and B receptors) and sarafotoxin 6c (a selective agonist for endothelin B receptors) in bronchial smooth muscle cells in Sephadex-induced inflammation, but not in tracheal smooth muscle cells. The enhanced maximal responses of bronchial smooth muscle cells to endothelin-1 and sarafotoxin 6c in Sephadex-induced inflammation support our molecular findings and hence imply a role for endothelin B receptors in airway hyperreactivity during airway inflammation.},
  author       = {Granström, Bengt and Xu, Cang-Bao and Nilsson, Elisabeth and Bengtsson, Ursula Hultkvist and Edvinsson, Lars},
  issn         = {1742-7843},
  language     = {eng},
  number       = {1},
  pages        = {43--48},
  publisher    = {Wiley-Blackwell},
  series       = {Basic & Clinical Pharmacology & Toxicology2004-01-01+01:002014-01-01+01:00},
  title        = {Up-regulation of endothelin receptor function and mRNA expression in airway smooth muscle cells following sephadex-induced airway inflammation.},
  url          = {http://dx.doi.org/10.1111/j.1742-7843.2004.pto950109.x},
  volume       = {95},
  year         = {2004},
}