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Antenna protein composition of PS I and PS II in thylakoid sub-domains

Jansson, Stefan; Stefánsson, Hreinn; Nyström, Ulrika; Gustafsson, Petter and Albertsson, Per-Åke LU (1997) In Biochimica et Biophysica Acta - Bioenergetics 1320(3). p.297-309
Abstract
Spinach thylakoids were separated into grana core, grana margin, and two different stromal lamella fractions in the absence of detergents. The levels of all light-harvesting chlorophyll a/b-binding (LHC) proteins were determined in all fractions, and were normalised to the amount of Photosystem I (PS I) and Photosystem II (PS II) centres. PS Iβ in the stroma lamellae was found to have a full complement of Lhca polypeptides and, probably, one attached LHC II trimer. PS Iα binds additional LHC II trimers, but PS I centres located in the inner parts of the grana stack lack Lhca1 and are depleted in Lhca4. PS IIβ, found in grana margins and stroma lamellae, seems to associate one monomer each of Lhcb4, Lhcb5 and Lhcb6 (CP29, CP26 and CP24,... (More)
Spinach thylakoids were separated into grana core, grana margin, and two different stromal lamella fractions in the absence of detergents. The levels of all light-harvesting chlorophyll a/b-binding (LHC) proteins were determined in all fractions, and were normalised to the amount of Photosystem I (PS I) and Photosystem II (PS II) centres. PS Iβ in the stroma lamellae was found to have a full complement of Lhca polypeptides and, probably, one attached LHC II trimer. PS Iα binds additional LHC II trimers, but PS I centres located in the inner parts of the grana stack lack Lhca1 and are depleted in Lhca4. PS IIβ, found in grana margins and stroma lamellae, seems to associate one monomer each of Lhcb4, Lhcb5 and Lhcb6 (CP29, CP26 and CP24, respectively) and one LHC II trimer consisting of two Lhcb1 and one Lhcb3 subunit. PS IIα has additional LHC II trimers (consisting of Lhcb1 and Lhcb2) attached. We also find evidence for the existence of both PS I and PS II centres in the extreme stroma (probably centres being synthesised or repaired), that lack all LHC proteins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Light-harvesting chlorophyll a/b-binding protein I, Light-harvesting chlorophyll a/b-binding protein II, Stroma exposed thylakoid, Grana margin, Grana, Heterogeneity
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1320
issue
3
pages
297 - 309
publisher
Elsevier
external identifiers
  • scopus:0342601367
ISSN
0005-2728
DOI
10.1016/S0005-2728(97)00033-9
language
English
LU publication?
yes
id
19ba1f5f-52eb-4025-b74b-266dde0be7c0 (old id 126169)
date added to LUP
2007-07-05 13:04:17
date last changed
2017-07-30 04:27:52
@article{19ba1f5f-52eb-4025-b74b-266dde0be7c0,
  abstract     = {Spinach thylakoids were separated into grana core, grana margin, and two different stromal lamella fractions in the absence of detergents. The levels of all light-harvesting chlorophyll a/b-binding (LHC) proteins were determined in all fractions, and were normalised to the amount of Photosystem I (PS I) and Photosystem II (PS II) centres. PS Iβ in the stroma lamellae was found to have a full complement of Lhca polypeptides and, probably, one attached LHC II trimer. PS Iα binds additional LHC II trimers, but PS I centres located in the inner parts of the grana stack lack Lhca1 and are depleted in Lhca4. PS IIβ, found in grana margins and stroma lamellae, seems to associate one monomer each of Lhcb4, Lhcb5 and Lhcb6 (CP29, CP26 and CP24, respectively) and one LHC II trimer consisting of two Lhcb1 and one Lhcb3 subunit. PS IIα has additional LHC II trimers (consisting of Lhcb1 and Lhcb2) attached. We also find evidence for the existence of both PS I and PS II centres in the extreme stroma (probably centres being synthesised or repaired), that lack all LHC proteins.},
  author       = {Jansson, Stefan and Stefánsson, Hreinn and Nyström, Ulrika and Gustafsson, Petter and Albertsson, Per-Åke},
  issn         = {0005-2728},
  keyword      = {Light-harvesting chlorophyll a/b-binding protein I,Light-harvesting chlorophyll a/b-binding protein II,Stroma exposed thylakoid,Grana margin,Grana,Heterogeneity},
  language     = {eng},
  number       = {3},
  pages        = {297--309},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Antenna protein composition of PS I and PS II in thylakoid sub-domains},
  url          = {http://dx.doi.org/10.1016/S0005-2728(97)00033-9},
  volume       = {1320},
  year         = {1997},
}