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Development of biosensors based on hexacyanoferrates.

de Mattos, I L; Gorton, Lo LU ; Laurell, Thomas LU ; Malinauskas, A and Karyakin, A A (2000) In Talanta 52(5). p.791-799
Abstract
Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were electrodeposited on glassy carbon electrodes providing a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Additionally glucose oxidase was immobilized on top of these electrodes to form glucose biosensors. The biosensors were made by casting glucose oxidase-Nafion layers onto the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution (5 muM H(2)O(2) for PB, 5 mM H(2)O(2) for CuHCF and 2.5 mM glucose for both) over 5-10 h in a flow-injection system with the electrodes polarized at -50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose... (More)
Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were electrodeposited on glassy carbon electrodes providing a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Additionally glucose oxidase was immobilized on top of these electrodes to form glucose biosensors. The biosensors were made by casting glucose oxidase-Nafion layers onto the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution (5 muM H(2)O(2) for PB, 5 mM H(2)O(2) for CuHCF and 2.5 mM glucose for both) over 5-10 h in a flow-injection system with the electrodes polarized at -50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose biosensors demonstrated suitability for glucose determination: 0.0-2.5 mM (R(2)=0.9977) for PB and 0.0-10 mM (R(2)=0.9927) for CuHCF, respectively. The visualization of the redox catalyst modifiers (PB and CuHCF films) was presented by scanning electron micrographs. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Talanta
volume
52
issue
5
pages
791 - 799
publisher
Elsevier
external identifiers
  • pmid:18968038
  • scopus:0034675042
ISSN
1873-3573
DOI
10.1016/S0039-9140(00)00409-4
language
English
LU publication?
yes
id
964385b3-fa4a-4038-be54-2fe61bd38c91 (old id 1261754)
date added to LUP
2008-12-01 10:41:27
date last changed
2017-04-09 04:16:26
@article{964385b3-fa4a-4038-be54-2fe61bd38c91,
  abstract     = {Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were electrodeposited on glassy carbon electrodes providing a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Additionally glucose oxidase was immobilized on top of these electrodes to form glucose biosensors. The biosensors were made by casting glucose oxidase-Nafion layers onto the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution (5 muM H(2)O(2) for PB, 5 mM H(2)O(2) for CuHCF and 2.5 mM glucose for both) over 5-10 h in a flow-injection system with the electrodes polarized at -50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose biosensors demonstrated suitability for glucose determination: 0.0-2.5 mM (R(2)=0.9977) for PB and 0.0-10 mM (R(2)=0.9927) for CuHCF, respectively. The visualization of the redox catalyst modifiers (PB and CuHCF films) was presented by scanning electron micrographs.},
  author       = {de Mattos, I L and Gorton, Lo and Laurell, Thomas and Malinauskas, A and Karyakin, A A},
  issn         = {1873-3573},
  language     = {eng},
  number       = {5},
  pages        = {791--799},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Development of biosensors based on hexacyanoferrates.},
  url          = {http://dx.doi.org/10.1016/S0039-9140(00)00409-4},
  volume       = {52},
  year         = {2000},
}