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Transient Aggregates in Protein Folding are Easily Mistaken for Folding Intermediates

Silow, Maria and Oliveberg, Mikael LU (1997) In Proceedings of the National Academy of Sciences 94(12). p.6084-6086
Abstract
It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biochemistry, U1A spliceosomal protein, Aggregation artefacts, Off-pathway intermediates
in
Proceedings of the National Academy of Sciences
volume
94
issue
12
pages
6084 - 6086
publisher
National Acad Sciences
external identifiers
  • scopus:0030958760
ISSN
1091-6490
DOI
10.1073/pnas.94.12.6084
language
English
LU publication?
yes
id
1954137a-f31c-4aae-9ca7-3c055c4ccd46 (old id 126214)
date added to LUP
2007-07-06 15:17:46
date last changed
2017-07-02 03:41:58
@article{1954137a-f31c-4aae-9ca7-3c055c4ccd46,
  abstract     = {It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.},
  author       = {Silow, Maria and Oliveberg, Mikael},
  issn         = {1091-6490},
  keyword      = {Biochemistry,U1A spliceosomal protein,Aggregation artefacts,Off-pathway intermediates},
  language     = {eng},
  number       = {12},
  pages        = {6084--6086},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Transient Aggregates in Protein Folding are Easily Mistaken for Folding Intermediates},
  url          = {http://dx.doi.org/10.1073/pnas.94.12.6084},
  volume       = {94},
  year         = {1997},
}