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Fractionation of the thylakoid membrane from Dunaliella salina – heterogeneity is found in Photosystem I over a broad range of growth irradiance

Stefánsson, Hreinn; Andreasson, Eva; Weibull, Claes LU and Albertsson, Per-Åke LU (1997) In Biochimica et Biophysica Acta - Bioenergetics 1320(3). p.235-246
Abstract
Thylakoids from the green alga, Dunaliella salina, were fragmented by sonication and the appressed grasna membranes separated from stroma lamellae by partitioning in aqueous two-phase systems. The concentration and antenna size of Photosystem I in the two membrane domains were determined for cultures grown at three different light intensities. Although the antenna size of both PS is decreased with increasing growth irradiance, the antenna size of Photosystem I in the grana was approximately 25-30% greater than the antenna size of Photosystem I in the stroma lamellae. Counter-current distribution analysis of sonicated thylakoids revealed that the amount of stroma lamellae increased whereas the amount of the stacked membranes decreased at... (More)
Thylakoids from the green alga, Dunaliella salina, were fragmented by sonication and the appressed grasna membranes separated from stroma lamellae by partitioning in aqueous two-phase systems. The concentration and antenna size of Photosystem I in the two membrane domains were determined for cultures grown at three different light intensities. Although the antenna size of both PS is decreased with increasing growth irradiance, the antenna size of Photosystem I in the grana was approximately 25-30% greater than the antenna size of Photosystem I in the stroma lamellae. Counter-current distribution analysis of sonicated thylakoids revealed that the amount of stroma lamellae increased whereas the amount of the stacked membranes decreased at higher growth irradiance. The overall decrease in the antenna size of PS I, in D. salina, at higher light intensities can therefore be explained by the combined effect of a decrease in the antenna size of Photosystem I, both in the grana and the stroma lamellae, and a relative increase in the amount of stroma lamellae which has smaller Photosystem I antennae than the Photosystem I centers found in the grana. Light-induced protein phosphorylation increased the relative amount of the stroma lamella fraction. This is interpreted as a result of partial unstacking of the grana. It is suggested that this may be a mechanism for increasing the cyclic electron transport around Photosystem I. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous two-phase partitioning, (Dunaliella salina), Membrane domain, Thylakoid membrane organization, Protein phosphorylation, Photosystem
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1320
issue
3
pages
235 - 246
publisher
Elsevier
external identifiers
  • scopus:0343051788
ISSN
0005-2728
DOI
10.1016/S0005-2728(97)00025-X
language
English
LU publication?
yes
id
09ae988b-366e-43e3-adbd-040073d62a1c (old id 126232)
date added to LUP
2007-07-06 15:32:29
date last changed
2017-01-01 07:00:07
@article{09ae988b-366e-43e3-adbd-040073d62a1c,
  abstract     = {Thylakoids from the green alga, Dunaliella salina, were fragmented by sonication and the appressed grasna membranes separated from stroma lamellae by partitioning in aqueous two-phase systems. The concentration and antenna size of Photosystem I in the two membrane domains were determined for cultures grown at three different light intensities. Although the antenna size of both PS is decreased with increasing growth irradiance, the antenna size of Photosystem I in the grana was approximately 25-30% greater than the antenna size of Photosystem I in the stroma lamellae. Counter-current distribution analysis of sonicated thylakoids revealed that the amount of stroma lamellae increased whereas the amount of the stacked membranes decreased at higher growth irradiance. The overall decrease in the antenna size of PS I, in D. salina, at higher light intensities can therefore be explained by the combined effect of a decrease in the antenna size of Photosystem I, both in the grana and the stroma lamellae, and a relative increase in the amount of stroma lamellae which has smaller Photosystem I antennae than the Photosystem I centers found in the grana. Light-induced protein phosphorylation increased the relative amount of the stroma lamella fraction. This is interpreted as a result of partial unstacking of the grana. It is suggested that this may be a mechanism for increasing the cyclic electron transport around Photosystem I.},
  author       = {Stefánsson, Hreinn and Andreasson, Eva and Weibull, Claes and Albertsson, Per-Åke},
  issn         = {0005-2728},
  keyword      = {Aqueous two-phase partitioning,(Dunaliella salina),Membrane domain,Thylakoid membrane organization,Protein phosphorylation,Photosystem},
  language     = {eng},
  number       = {3},
  pages        = {235--246},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Fractionation of the thylakoid membrane from Dunaliella salina – heterogeneity is found in Photosystem I over a broad range of growth irradiance},
  url          = {http://dx.doi.org/10.1016/S0005-2728(97)00025-X},
  volume       = {1320},
  year         = {1997},
}