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Crystallization and preliminary X-ray analysis of human dUTPase

Larsson, G; Nyman, Per-Olof LU and Svensson, L A (1996) In Acta Crystallographica. Section D: Biological Crystallography D52(5). p.1039-1040
Abstract
Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section D: Biological Crystallography
volume
D52
issue
5
pages
1039 - 1040
publisher
International Union of Crystallography
external identifiers
  • scopus:0030513996
ISSN
1399-0047
DOI
10.1107/S0907444996003277
language
English
LU publication?
yes
id
0aa1f215-5b3c-4bf1-9041-e1aa1c2d2f1a (old id 126300)
date added to LUP
2007-07-06 10:01:49
date last changed
2017-01-01 07:05:45
@article{0aa1f215-5b3c-4bf1-9041-e1aa1c2d2f1a,
  abstract     = {Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.},
  author       = {Larsson, G and Nyman, Per-Olof and Svensson, L A},
  issn         = {1399-0047},
  language     = {eng},
  number       = {5},
  pages        = {1039--1040},
  publisher    = {International Union of Crystallography},
  series       = {Acta Crystallographica. Section D: Biological Crystallography},
  title        = {Crystallization and preliminary X-ray analysis of human dUTPase},
  url          = {http://dx.doi.org/10.1107/S0907444996003277},
  volume       = {D52},
  year         = {1996},
}