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Decomplexing biofluids using microchip based acoustophoresis

Augustsson, Per LU ; Persson, Jonas LU ; Ekström, Simon LU ; Ohlin, Mats LU and Laurell, Thomas LU (2009) In Lab on A Chip 9(6). p.810-818
Abstract
Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart... (More)
Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart is demonstrated using metal oxide affinity capture (MOAC) beads and MALDI-TOF MS readout. Acoustophoresis of microbeads activated with specific binders could be used to capture phage viral particles. The efficiency of the acoustophoretic washing principle was demonstrated by an unspecific phage cross contamination level of only 10(-6) of that in the input bead/phage mixture. The continuous flow format makes acoustophoretic washing flexible regarding sample volume and also allows for easy integration into a sequence of particle handling and analytical unit operations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
9
issue
6
pages
810 - 818
publisher
Royal Society of Chemistry
external identifiers
  • wos:000263847000009
  • pmid:19255663
  • scopus:61849089803
ISSN
1473-0189
DOI
10.1039/B811027A
project
CREATE Health
language
English
LU publication?
yes
id
a2a63fe1-5922-4ca7-89d9-7281b2d6f44d (old id 1265242)
date added to LUP
2008-11-06 11:23:01
date last changed
2017-09-24 03:33:23
@article{a2a63fe1-5922-4ca7-89d9-7281b2d6f44d,
  abstract     = {Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart is demonstrated using metal oxide affinity capture (MOAC) beads and MALDI-TOF MS readout. Acoustophoresis of microbeads activated with specific binders could be used to capture phage viral particles. The efficiency of the acoustophoretic washing principle was demonstrated by an unspecific phage cross contamination level of only 10(-6) of that in the input bead/phage mixture. The continuous flow format makes acoustophoretic washing flexible regarding sample volume and also allows for easy integration into a sequence of particle handling and analytical unit operations.},
  author       = {Augustsson, Per and Persson, Jonas and Ekström, Simon and Ohlin, Mats and Laurell, Thomas},
  issn         = {1473-0189},
  language     = {eng},
  number       = {6},
  pages        = {810--818},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Decomplexing biofluids using microchip based acoustophoresis},
  url          = {http://dx.doi.org/10.1039/B811027A},
  volume       = {9},
  year         = {2009},
}