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Expression of Alkaline Sphingomyelinase in Yeast Cells and Anti-inflammatory Effects of the Expressed Enzyme in a Rat Colitis Model.

Andersson, David LU ; Kotarsky, Knut LU ; Wu, Jun LU ; Agace, William LU and Duan, Rui-Dong LU (2009) In Digestive Diseases and Sciences 2008(Nov 7). p.1440-1448
Abstract
Alkaline sphingomyelinase (Alk-SMase) is a key enzyme in the intestinal tract for digestion of dietary sphingomyelin (SM), which generates lipid messengers with cell-cycle regulating effects. The enzyme is significantly decreased in ulcerative colitis and colon cancer. Based on this information, we wanted to investigate whether the enzyme had preventive effects against murine colitis. We report herein a method to express a biologically active Alk-SMase from Pichia pastoris yeast cells. By using the expressed enzyme to treat a rat colitis model induced by dextran sulfate sodium, we found that intrarectal instillation of Alk-SMase once daily for 1 week significantly reduced the inflammation score and protected the colonic epithelium from... (More)
Alkaline sphingomyelinase (Alk-SMase) is a key enzyme in the intestinal tract for digestion of dietary sphingomyelin (SM), which generates lipid messengers with cell-cycle regulating effects. The enzyme is significantly decreased in ulcerative colitis and colon cancer. Based on this information, we wanted to investigate whether the enzyme had preventive effects against murine colitis. We report herein a method to express a biologically active Alk-SMase from Pichia pastoris yeast cells. By using the expressed enzyme to treat a rat colitis model induced by dextran sulfate sodium, we found that intrarectal instillation of Alk-SMase once daily for 1 week significantly reduced the inflammation score and protected the colonic epithelium from inflammatory destruction. We found a tendency for decreased tumor necrosis factor (TNF)-alpha expression in the Alk-SMase-treated group. This study, for the first time, provides a method to produce the enzyme and shows the potential applicability of the enzyme in the treatment of inflammatory bowel diseases. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Digestive Diseases and Sciences
volume
2008
issue
Nov 7
pages
1440 - 1448
publisher
Springer
external identifiers
  • wos:000266654900008
  • pmid:18989780
  • scopus:67349153090
  • pmid:18989780
ISSN
1573-2568
DOI
10.1007/s10620-008-0509-2
language
English
LU publication?
yes
id
516cf456-cf57-4b04-b8f6-e0c2820f8d1a (old id 1271770)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18989780?dopt=Abstract
date added to LUP
2016-04-01 12:06:28
date last changed
2024-10-08 21:58:11
@article{516cf456-cf57-4b04-b8f6-e0c2820f8d1a,
  abstract     = {{Alkaline sphingomyelinase (Alk-SMase) is a key enzyme in the intestinal tract for digestion of dietary sphingomyelin (SM), which generates lipid messengers with cell-cycle regulating effects. The enzyme is significantly decreased in ulcerative colitis and colon cancer. Based on this information, we wanted to investigate whether the enzyme had preventive effects against murine colitis. We report herein a method to express a biologically active Alk-SMase from Pichia pastoris yeast cells. By using the expressed enzyme to treat a rat colitis model induced by dextran sulfate sodium, we found that intrarectal instillation of Alk-SMase once daily for 1 week significantly reduced the inflammation score and protected the colonic epithelium from inflammatory destruction. We found a tendency for decreased tumor necrosis factor (TNF)-alpha expression in the Alk-SMase-treated group. This study, for the first time, provides a method to produce the enzyme and shows the potential applicability of the enzyme in the treatment of inflammatory bowel diseases.}},
  author       = {{Andersson, David and Kotarsky, Knut and Wu, Jun and Agace, William and Duan, Rui-Dong}},
  issn         = {{1573-2568}},
  language     = {{eng}},
  number       = {{Nov 7}},
  pages        = {{1440--1448}},
  publisher    = {{Springer}},
  series       = {{Digestive Diseases and Sciences}},
  title        = {{Expression of Alkaline Sphingomyelinase in Yeast Cells and Anti-inflammatory Effects of the Expressed Enzyme in a Rat Colitis Model.}},
  url          = {{http://dx.doi.org/10.1007/s10620-008-0509-2}},
  doi          = {{10.1007/s10620-008-0509-2}},
  volume       = {{2008}},
  year         = {{2009}},
}