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Acoustic Standing Wave Manipulation of Particles and Cells in Microfluidic Chips

Lenshof, Andreas LU (2009)
Abstract
The rise of MEMS and µTAS techniques has created a whole new family of microfluidic devices for a wide range of chemical and biomedical analyses to be performed on small Lab-on-a-chip platforms. The operations often include small samples of particle or cell suspensions on which separation, mixing, trapping or sorting is performed. External fields and forces are used for these operations, and this thesis is specifically focused the development of ultrasonic standing wave technology and the use of acoustic force fields to perform bioanalytical unit operations.

The combination of acoustic standing waves and the laminar flow in microfluidics has proven to be well suited for performing particle and cell separation. The fundamental... (More)
The rise of MEMS and µTAS techniques has created a whole new family of microfluidic devices for a wide range of chemical and biomedical analyses to be performed on small Lab-on-a-chip platforms. The operations often include small samples of particle or cell suspensions on which separation, mixing, trapping or sorting is performed. External fields and forces are used for these operations, and this thesis is specifically focused the development of ultrasonic standing wave technology and the use of acoustic force fields to perform bioanalytical unit operations.

The combination of acoustic standing waves and the laminar flow in microfluidics has proven to be well suited for performing particle and cell separation. The fundamental acoustic separator used in this thesis consists of a microfluidic flow channel with a three way flow splitter (trifurcation) in the end of the channel. An acoustic standing wave field is applied to the main flow channel by attaching the transducer underneath the chip. The acoustic standing wave is however obtained perpendicular to the axial propagation of the wave field and the direction of the flow. The half wavelength resonance affects rigid particles or cells driving them into the acoustic pressure node while liquid spheres having other density and compressibility properties may move to the pressure antinode. This enables acoustic separation of different particle types. Blood has proven to be very suitable for acoustic cell manipulation. An application where lipid particles can be removed acoustically from shed blood from open heart surgery is demonstrated. An application for acoustic plasmapheresis is also shown where high quality blood plasma is generated. Different separator designs, device material, and the influence of the separation channel cross-section design are also investigated. (Less)
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author
supervisor
opponent
  • Dr Pamme, Nicole, Dept. of Chemistry, University of Hull, Hull, United Kingdom
organization
publishing date
type
Thesis
publication status
published
subject
keywords
acoustic particle manipulation, separation, ultrasound, cell handling, particle handling, lab on a chip, Microsystem technology, microfluidics, standing waves
pages
146 pages
publisher
Lund University
defense location
Room E:1406, E-building, John Erikssons väg 4, Lund University, Faculty of Engineering
defense date
2009-01-30 10:15
ISSN
0346-6221
ISBN
978-91-628-7678-4
language
English
LU publication?
yes
id
742c4fbe-0a76-4261-aaee-cf3c66e82b7f (old id 1275328)
date added to LUP
2008-12-29 11:17:40
date last changed
2016-09-19 08:44:46
@phdthesis{742c4fbe-0a76-4261-aaee-cf3c66e82b7f,
  abstract     = {The rise of MEMS and µTAS techniques has created a whole new family of microfluidic devices for a wide range of chemical and biomedical analyses to be performed on small Lab-on-a-chip platforms. The operations often include small samples of particle or cell suspensions on which separation, mixing, trapping or sorting is performed. External fields and forces are used for these operations, and this thesis is specifically focused the development of ultrasonic standing wave technology and the use of acoustic force fields to perform bioanalytical unit operations.<br/><br>
The combination of acoustic standing waves and the laminar flow in microfluidics has proven to be well suited for performing particle and cell separation. The fundamental acoustic separator used in this thesis consists of a microfluidic flow channel with a three way flow splitter (trifurcation) in the end of the channel. An acoustic standing wave field is applied to the main flow channel by attaching the transducer underneath the chip. The acoustic standing wave is however obtained perpendicular to the axial propagation of the wave field and the direction of the flow. The half wavelength resonance affects rigid particles or cells driving them into the acoustic pressure node while liquid spheres having other density and compressibility properties may move to the pressure antinode. This enables acoustic separation of different particle types. Blood has proven to be very suitable for acoustic cell manipulation. An application where lipid particles can be removed acoustically from shed blood from open heart surgery is demonstrated. An application for acoustic plasmapheresis is also shown where high quality blood plasma is generated. Different separator designs, device material, and the influence of the separation channel cross-section design are also investigated.},
  author       = {Lenshof, Andreas},
  isbn         = {978-91-628-7678-4},
  issn         = {0346-6221},
  keyword      = {acoustic particle manipulation,separation,ultrasound,cell handling,particle handling,lab on a chip,Microsystem technology,microfluidics,standing waves},
  language     = {eng},
  pages        = {146},
  publisher    = {Lund University},
  school       = {Lund University},
  title        = {Acoustic Standing Wave Manipulation of Particles and Cells in Microfluidic Chips},
  year         = {2009},
}