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Proteasome-dependent degradation of ERalpha but not ERbeta in cultured mouse aorta smooth muscle cells.

Liang, Min LU and Nilsson, Bengt-Olof LU (2004) In Molecular and Cellular Endocrinology 224(1-2). p.65-71
Abstract
Here we investigate ERα and ERβ expression and regulation in vascular smooth muscle cells from mouse aorta. Immunocytochemistry showed nuclear staining for both ERα and ERβ. Double stainings revealed co-expression of ERα and ERβ in vascular smooth muscle cells. ERα (66 kDa) and ERβ (54 kDa) expression determined by Western blotting was unchanged within 7 h after inhibition of protein synthesis with cycloheximide in the absence of 17β-estradiol (E2), showing that both proteins are stable without ligand-binding. Treatment with 10 nM E2 for 7 h in the presence of cycloheximide increased ERα, suggesting that E2 causes a conformational change in the ERα protein. The ERβ was not affected by E2. Treatment with the proteasome inhibitor epoxomicin... (More)
Here we investigate ERα and ERβ expression and regulation in vascular smooth muscle cells from mouse aorta. Immunocytochemistry showed nuclear staining for both ERα and ERβ. Double stainings revealed co-expression of ERα and ERβ in vascular smooth muscle cells. ERα (66 kDa) and ERβ (54 kDa) expression determined by Western blotting was unchanged within 7 h after inhibition of protein synthesis with cycloheximide in the absence of 17β-estradiol (E2), showing that both proteins are stable without ligand-binding. Treatment with 10 nM E2 for 7 h in the presence of cycloheximide increased ERα, suggesting that E2 causes a conformational change in the ERα protein. The ERβ was not affected by E2. Treatment with the proteasome inhibitor epoxomicin (100 nM) for 3 days caused a prominent upregulation of ERα both in the absence and in the presence of E2, while ERβ was unaffected, suggesting that ERα but not ERβ is degraded by ubiquitin–proteasome system in vascular smooth muscle cells. In summary, we disclose a short-term regulation of ERα protein by estrogen and that ERα but not ERβ is degraded via the ubiquitin–proteasome pathway in vascular smooth muscle cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immunocytochemistry, Estrogen receptor α and β, Estrogen, Vascular smooth muscle cells, Western blotting
in
Molecular and Cellular Endocrinology
volume
224
issue
1-2
pages
65 - 71
publisher
Elsevier
external identifiers
  • pmid:15353181
  • wos:000224370400007
  • scopus:4444285037
ISSN
1872-8057
DOI
10.1016/j.mce.2004.06.012
language
English
LU publication?
yes
id
d79b16f4-568a-4b57-a959-207a72866007 (old id 127543)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15353181&dopt=Abstract
date added to LUP
2007-07-16 15:38:13
date last changed
2017-10-01 03:53:25
@article{d79b16f4-568a-4b57-a959-207a72866007,
  abstract     = {Here we investigate ERα and ERβ expression and regulation in vascular smooth muscle cells from mouse aorta. Immunocytochemistry showed nuclear staining for both ERα and ERβ. Double stainings revealed co-expression of ERα and ERβ in vascular smooth muscle cells. ERα (66 kDa) and ERβ (54 kDa) expression determined by Western blotting was unchanged within 7 h after inhibition of protein synthesis with cycloheximide in the absence of 17β-estradiol (E2), showing that both proteins are stable without ligand-binding. Treatment with 10 nM E2 for 7 h in the presence of cycloheximide increased ERα, suggesting that E2 causes a conformational change in the ERα protein. The ERβ was not affected by E2. Treatment with the proteasome inhibitor epoxomicin (100 nM) for 3 days caused a prominent upregulation of ERα both in the absence and in the presence of E2, while ERβ was unaffected, suggesting that ERα but not ERβ is degraded by ubiquitin–proteasome system in vascular smooth muscle cells. In summary, we disclose a short-term regulation of ERα protein by estrogen and that ERα but not ERβ is degraded via the ubiquitin–proteasome pathway in vascular smooth muscle cells.},
  author       = {Liang, Min and Nilsson, Bengt-Olof},
  issn         = {1872-8057},
  keyword      = {Immunocytochemistry,Estrogen receptor α and β,Estrogen,Vascular smooth muscle cells,Western blotting},
  language     = {eng},
  number       = {1-2},
  pages        = {65--71},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Endocrinology},
  title        = {Proteasome-dependent degradation of ERalpha but not ERbeta in cultured mouse aorta smooth muscle cells.},
  url          = {http://dx.doi.org/10.1016/j.mce.2004.06.012},
  volume       = {224},
  year         = {2004},
}