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Implantation of Schwann cells in rat tendon autografts as a model for peripheral nerve repair: Long term effects on functional recovery

Arino, Hiroshi; Brandt, Jerker LU and Dahlin, Lars LU (2008) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 42(6). p.281-285
Abstract
Cultured Schwann cells in tendon autografts for nerve repair improve the early phase of nerve regeneration in rat sciatic nerves as judged by the rate of axonal outgrowth. We tested the long-term effects on functional recovery using measurements of muscle force, the number of axons and myelination, using morphometry. In addition, we recorded wet weight of the gastrocnemius muscle. Schwann cell cultures were prepared from predegenerated nerves. Ten and 15mm defects in rat sciatic nerves were bridged using bilateral tendon autografts with Schwann cell-seeded tendon autografts on one side, and untreated tendon autografts on the other. Animals were evaluated at six and 12 weeks, respectively. At six weeks, myelination, as judged by G-ratio... (More)
Cultured Schwann cells in tendon autografts for nerve repair improve the early phase of nerve regeneration in rat sciatic nerves as judged by the rate of axonal outgrowth. We tested the long-term effects on functional recovery using measurements of muscle force, the number of axons and myelination, using morphometry. In addition, we recorded wet weight of the gastrocnemius muscle. Schwann cell cultures were prepared from predegenerated nerves. Ten and 15mm defects in rat sciatic nerves were bridged using bilateral tendon autografts with Schwann cell-seeded tendon autografts on one side, and untreated tendon autografts on the other. Animals were evaluated at six and 12 weeks, respectively. At six weeks, myelination, as judged by G-ratio (ratio of axonal diameter to diameter of nerve fibres), was significantly increased in tendon autografts pretreated with Schwann cells in 10mm defects. No such difference was seen in the 15 mmdefects. We found no difference in functional recovery, other morphometric variables, or muscle weight between the two grafts. We conclude that early effects on nerve regeneration using transplantation of cultured Schwann cells in rat sciatic nerves are temporary. Other strategies are necessary to obtain lasting effects on functional recovery. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nerve regeneration, morphometry, muscle force, functional recovery, Schwann cell, tendon autograft, transplantation
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
volume
42
issue
6
pages
281 - 285
publisher
Taylor & Francis
external identifiers
  • wos:000260768400001
  • scopus:57349090211
ISSN
1651-2073
DOI
10.1080/02844310802393966
language
English
LU publication?
yes
id
01560911-a3b4-4e30-bd06-f09aa71ffa48 (old id 1278965)
date added to LUP
2009-02-10 13:08:30
date last changed
2017-02-19 03:52:54
@article{01560911-a3b4-4e30-bd06-f09aa71ffa48,
  abstract     = {Cultured Schwann cells in tendon autografts for nerve repair improve the early phase of nerve regeneration in rat sciatic nerves as judged by the rate of axonal outgrowth. We tested the long-term effects on functional recovery using measurements of muscle force, the number of axons and myelination, using morphometry. In addition, we recorded wet weight of the gastrocnemius muscle. Schwann cell cultures were prepared from predegenerated nerves. Ten and 15mm defects in rat sciatic nerves were bridged using bilateral tendon autografts with Schwann cell-seeded tendon autografts on one side, and untreated tendon autografts on the other. Animals were evaluated at six and 12 weeks, respectively. At six weeks, myelination, as judged by G-ratio (ratio of axonal diameter to diameter of nerve fibres), was significantly increased in tendon autografts pretreated with Schwann cells in 10mm defects. No such difference was seen in the 15 mmdefects. We found no difference in functional recovery, other morphometric variables, or muscle weight between the two grafts. We conclude that early effects on nerve regeneration using transplantation of cultured Schwann cells in rat sciatic nerves are temporary. Other strategies are necessary to obtain lasting effects on functional recovery.},
  author       = {Arino, Hiroshi and Brandt, Jerker and Dahlin, Lars},
  issn         = {1651-2073},
  keyword      = {nerve regeneration,morphometry,muscle force,functional recovery,Schwann cell,tendon autograft,transplantation},
  language     = {eng},
  number       = {6},
  pages        = {281--285},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery},
  title        = {Implantation of Schwann cells in rat tendon autografts as a model for peripheral nerve repair: Long term effects on functional recovery},
  url          = {http://dx.doi.org/10.1080/02844310802393966},
  volume       = {42},
  year         = {2008},
}