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The Yersinia HPI is present in Serratia liquefaciens isolated from meat.

Olsson, Crister LU ; Olofsson, T; Ahrné, Siv LU and Molin, Göran LU (2003) In Letters in Applied Microbiology 37(4). p.275-280
Abstract
Aims: The aim of the study was to screen the Enterobacteriaceae flora of meat for the presence of bacteria harbouring the Yersinia high-pathogenicity island (HPI).



Methods and Results: Bacteria from 29 meat and 29 liver samples were isolated on violetred bile glucose agar. A total of 197 isolates were screened for the presence of the irp2 gene, encoded within the HPI, by PCR. One isolate that was positive for irp2 gene was also positive for the fyuA, irp1, ybtP/ybtQ, ybtX/ybtS and int/asn tRNA genes by PCR. The presence of fyuA, irp1 and irp2 genes was confirmed by Southern hybridization.



Conclusions: The isolate was identified as Serratia liquefaciens by sequencing of the 16S rRNA gene and by... (More)
Aims: The aim of the study was to screen the Enterobacteriaceae flora of meat for the presence of bacteria harbouring the Yersinia high-pathogenicity island (HPI).



Methods and Results: Bacteria from 29 meat and 29 liver samples were isolated on violetred bile glucose agar. A total of 197 isolates were screened for the presence of the irp2 gene, encoded within the HPI, by PCR. One isolate that was positive for irp2 gene was also positive for the fyuA, irp1, ybtP/ybtQ, ybtX/ybtS and int/asn tRNA genes by PCR. The presence of fyuA, irp1 and irp2 genes was confirmed by Southern hybridization.



Conclusions: The isolate was identified as Serratia liquefaciens by sequencing of the 16S rRNA gene and by ribotyping.



Significance and Impact of the Study: This is the first report of a Serratia harbouring the Yersinia HPI. Serratia is a frequently occurring Enterobacteriaceae genus in chill-stored meat. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Letters in Applied Microbiology
volume
37
issue
4
pages
275 - 280
publisher
Wiley-Blackwell
external identifiers
  • pmid:12969488
  • wos:000185225600001
  • scopus:0141650517
ISSN
0266-8254
DOI
language
English
LU publication?
yes
id
4148477d-33ff-4087-8d7f-ea898da3c06d (old id 128301)
date added to LUP
2007-07-19 09:02:17
date last changed
2018-05-29 10:41:56
@article{4148477d-33ff-4087-8d7f-ea898da3c06d,
  abstract     = {Aims: The aim of the study was to screen the Enterobacteriaceae flora of meat for the presence of bacteria harbouring the Yersinia high-pathogenicity island (HPI).<br/><br>
<br/><br>
Methods and Results: Bacteria from 29 meat and 29 liver samples were isolated on violetred bile glucose agar. A total of 197 isolates were screened for the presence of the irp2 gene, encoded within the HPI, by PCR. One isolate that was positive for irp2 gene was also positive for the fyuA, irp1, ybtP/ybtQ, ybtX/ybtS and int/asn tRNA genes by PCR. The presence of fyuA, irp1 and irp2 genes was confirmed by Southern hybridization.<br/><br>
<br/><br>
Conclusions: The isolate was identified as Serratia liquefaciens by sequencing of the 16S rRNA gene and by ribotyping.<br/><br>
<br/><br>
Significance and Impact of the Study: This is the first report of a Serratia harbouring the Yersinia HPI. Serratia is a frequently occurring Enterobacteriaceae genus in chill-stored meat.},
  author       = {Olsson, Crister and Olofsson, T and Ahrné, Siv and Molin, Göran},
  issn         = {0266-8254},
  language     = {eng},
  number       = {4},
  pages        = {275--280},
  publisher    = {Wiley-Blackwell},
  series       = {Letters in Applied Microbiology},
  title        = {The Yersinia HPI is present in Serratia liquefaciens isolated from meat.},
  url          = {http://dx.doi.org/},
  volume       = {37},
  year         = {2003},
}