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Changes in pore morphology and fluid transport in compressed articular cartilage and the implications for joint lubrication

Greene, George W.; Zappone, Bruno; Zhao, Boxin; Söderman, Olle LU ; Topgaard, Daniel LU ; Rata, Gabriel LU and Israelachvili, Jacob N. (2008) In Biomaterials 29(33). p.4455-4462
Abstract
Cartilage sections were cut from the middle zone of pig knee articular cartilage and attached to substrates in two different kinds of newly designed 'pressure cells', one for fluorescence the other for NMR measurements, The fluorescence cell was filled with buffer solution containing fluorescently marked 70 kDa dextran which was allowed to diffuse into the cartilage pores. A second glass surface was then pressed down onto the thin cartilage sample under different loads (pressures), and the resulting compression (strain) and change in pore volume were measured as a function of time, simultaneously with measurements of the lateral diffusion and flow pattern of the dextran molecules using Fluorescence Recovery After Photobleaching (FRAP).... (More)
Cartilage sections were cut from the middle zone of pig knee articular cartilage and attached to substrates in two different kinds of newly designed 'pressure cells', one for fluorescence the other for NMR measurements, The fluorescence cell was filled with buffer solution containing fluorescently marked 70 kDa dextran which was allowed to diffuse into the cartilage pores. A second glass surface was then pressed down onto the thin cartilage sample under different loads (pressures), and the resulting compression (strain) and change in pore volume were measured as a function of time, simultaneously with measurements of the lateral diffusion and flow pattern of the dextran molecules using Fluorescence Recovery After Photobleaching (FRAP). Complementary experiments were made on the normal diffusion coefficients of pure electrolyte solutions (no dextran) in thicker cartilage sections with pulse-gradient NMR using a new pressure cell suitable for such measurements. Taken together our results show that the highly anisotropic structure of cartilage has a strong effect on the way fluid diffuses laterally and normally at different stages of compression. Our results also show how geometric constraints on a cartilage network and trapped high MW polymer such as HA during normal compressions are likely to affect both the normal and the lateral mobilities of polyelectrolytes and water. (C) 2008 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Collagen structure, Pulse-gradient NMR, FRAP, Cartilage, Fluorescence recovery after photobleaching, Joint lubrication
in
Biomaterials
volume
29
issue
33
pages
4455 - 4462
publisher
Elsevier
external identifiers
  • wos:000260158100011
  • scopus:50849142847
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2008.07.046
language
English
LU publication?
yes
id
4f0b7dc2-6988-41f5-a039-e32b876b6b79 (old id 1284996)
date added to LUP
2009-02-06 11:12:25
date last changed
2017-05-21 03:34:26
@article{4f0b7dc2-6988-41f5-a039-e32b876b6b79,
  abstract     = {Cartilage sections were cut from the middle zone of pig knee articular cartilage and attached to substrates in two different kinds of newly designed 'pressure cells', one for fluorescence the other for NMR measurements, The fluorescence cell was filled with buffer solution containing fluorescently marked 70 kDa dextran which was allowed to diffuse into the cartilage pores. A second glass surface was then pressed down onto the thin cartilage sample under different loads (pressures), and the resulting compression (strain) and change in pore volume were measured as a function of time, simultaneously with measurements of the lateral diffusion and flow pattern of the dextran molecules using Fluorescence Recovery After Photobleaching (FRAP). Complementary experiments were made on the normal diffusion coefficients of pure electrolyte solutions (no dextran) in thicker cartilage sections with pulse-gradient NMR using a new pressure cell suitable for such measurements. Taken together our results show that the highly anisotropic structure of cartilage has a strong effect on the way fluid diffuses laterally and normally at different stages of compression. Our results also show how geometric constraints on a cartilage network and trapped high MW polymer such as HA during normal compressions are likely to affect both the normal and the lateral mobilities of polyelectrolytes and water. (C) 2008 Elsevier Ltd. All rights reserved.},
  author       = {Greene, George W. and Zappone, Bruno and Zhao, Boxin and Söderman, Olle and Topgaard, Daniel and Rata, Gabriel and Israelachvili, Jacob N.},
  issn         = {1878-5905},
  keyword      = {Collagen structure,Pulse-gradient NMR,FRAP,Cartilage,Fluorescence recovery after photobleaching,Joint lubrication},
  language     = {eng},
  number       = {33},
  pages        = {4455--4462},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Changes in pore morphology and fluid transport in compressed articular cartilage and the implications for joint lubrication},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2008.07.046},
  volume       = {29},
  year         = {2008},
}