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Enhanced Ribosome and tRNA Contents in Escherichia coli Expressing a Truncated Vitreoscilla Hemoglobin Mutant Analyzed by Flow Field-Flow Fractionation

Andersson, Charlotte LU ; Arfvidsson, Cecilia LU ; Kallio, P T; Wahlund, Karl-Gustav LU and Bülow, Leif LU (2003) In Biotechnology Letters 25(18). p.1499-1504
Abstract
The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the... (More)
The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the mass fraction of active 70S ribosomes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
25
issue
18
pages
1499 - 1504
publisher
Springer
external identifiers
  • pmid:14571972
  • wos:000184957400003
  • scopus:0141789646
ISSN
1573-6776
DOI
10.1023/A:1025475703006
language
English
LU publication?
yes
id
5c230f1c-dd3d-46b8-ada3-76e93b1afa16 (old id 128589)
date added to LUP
2007-06-26 11:39:24
date last changed
2018-05-29 09:57:19
@article{5c230f1c-dd3d-46b8-ada3-76e93b1afa16,
  abstract     = {The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the mass fraction of active 70S ribosomes.},
  author       = {Andersson, Charlotte and Arfvidsson, Cecilia and Kallio, P T and Wahlund, Karl-Gustav and Bülow, Leif},
  issn         = {1573-6776},
  language     = {eng},
  number       = {18},
  pages        = {1499--1504},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Enhanced Ribosome and tRNA Contents in Escherichia coli Expressing a Truncated Vitreoscilla Hemoglobin Mutant Analyzed by Flow Field-Flow Fractionation},
  url          = {http://dx.doi.org/10.1023/A:1025475703006},
  volume       = {25},
  year         = {2003},
}