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Antagonistic and synergistic effects of fungal and bacterial growth in soil after adding different carbon and nitrogen sources

Meidute, Sandra LU ; Demoling, Fredrik LU and Bååth, Erland LU (2008) In Soil Biology & Biochemistry 40(9). p.2334-2343
Abstract
The effect of adding easily available and more complex carbon sources, with and without nitrogen, on fungal and bacterial growth and activity in soil were studied in the laboratory. Total microbial activity was estimated by measuring respiration, fungal growth with the acetate-in-ergosterol incorporation technique and bacterial growth with the thymidine and leucine incorporation techniques. The substrate additions consisted of glucose and cellulose, with and without nitrogen (as ammonium nitrate), and gelatine. The microbial development was followed over a 2-month period. The respiration rate increased within a few days after adding glucose, with and without nitrogen, and gelatine, initially by more than 10 but after 2 months no... (More)
The effect of adding easily available and more complex carbon sources, with and without nitrogen, on fungal and bacterial growth and activity in soil were studied in the laboratory. Total microbial activity was estimated by measuring respiration, fungal growth with the acetate-in-ergosterol incorporation technique and bacterial growth with the thymidine and leucine incorporation techniques. The substrate additions consisted of glucose and cellulose, with and without nitrogen (as ammonium nitrate), and gelatine. The microbial development was followed over a 2-month period. The respiration rate increased within a few days after adding glucose, with and without nitrogen, and gelatine, initially by more than 10 but after 2 months no differences were seen compared with the control. Bacterial growth estimated, mated with the thymidine and leucine incorporation techniques gave similar results. Adding glucose with nitrogen, or gelatine, increased bacterial growth within a few days up to 10 times, but even after 2 months of incubation bacterial growth rates were still about 5 times higher than in the control. Adding only glucose increased bacterial growth rates by about twice over the whole incubation period. Fungal growth rates especially increased after adding cellulose and nitrogen, although a minor increase was found after adding cellulose alone. Fungal growth rates started to increase after 10 days of incubation with cellulose. There were indications of synergistic effects in that bacterial growth increased after the fungi had started to grow after adding cellulose. Treatments resulting in high bacterial growth rates (adding easily available carbon sources) led to decreased fungal growth rates compared with the control, indicating antagonistic effects of bacteria. (C) 2008 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
C-14 acetate, leucine, thymidine, fungi, bacteria, ergosterol
in
Soil Biology & Biochemistry
volume
40
issue
9
pages
2334 - 2343
publisher
Elsevier
external identifiers
  • wos:000259341500035
  • scopus:49249120060
ISSN
0038-0717
DOI
10.1016/j.soilbio.2008.05.011
language
English
LU publication?
yes
id
f2386826-3812-482e-b991-25ba1aa5b723 (old id 1286953)
date added to LUP
2009-01-28 10:54:26
date last changed
2017-09-24 04:07:31
@article{f2386826-3812-482e-b991-25ba1aa5b723,
  abstract     = {The effect of adding easily available and more complex carbon sources, with and without nitrogen, on fungal and bacterial growth and activity in soil were studied in the laboratory. Total microbial activity was estimated by measuring respiration, fungal growth with the acetate-in-ergosterol incorporation technique and bacterial growth with the thymidine and leucine incorporation techniques. The substrate additions consisted of glucose and cellulose, with and without nitrogen (as ammonium nitrate), and gelatine. The microbial development was followed over a 2-month period. The respiration rate increased within a few days after adding glucose, with and without nitrogen, and gelatine, initially by more than 10 but after 2 months no differences were seen compared with the control. Bacterial growth estimated, mated with the thymidine and leucine incorporation techniques gave similar results. Adding glucose with nitrogen, or gelatine, increased bacterial growth within a few days up to 10 times, but even after 2 months of incubation bacterial growth rates were still about 5 times higher than in the control. Adding only glucose increased bacterial growth rates by about twice over the whole incubation period. Fungal growth rates especially increased after adding cellulose and nitrogen, although a minor increase was found after adding cellulose alone. Fungal growth rates started to increase after 10 days of incubation with cellulose. There were indications of synergistic effects in that bacterial growth increased after the fungi had started to grow after adding cellulose. Treatments resulting in high bacterial growth rates (adding easily available carbon sources) led to decreased fungal growth rates compared with the control, indicating antagonistic effects of bacteria. (C) 2008 Elsevier Ltd. All rights reserved.},
  author       = {Meidute, Sandra and Demoling, Fredrik and Bååth, Erland},
  issn         = {0038-0717},
  keyword      = {C-14 acetate,leucine,thymidine,fungi,bacteria,ergosterol},
  language     = {eng},
  number       = {9},
  pages        = {2334--2343},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Antagonistic and synergistic effects of fungal and bacterial growth in soil after adding different carbon and nitrogen sources},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2008.05.011},
  volume       = {40},
  year         = {2008},
}