Advanced

Genetic Diversity Patterns in Five Protist Species Occurring in Lakes.

Logares, Ramiro LU ; Boltovskoy, Andrés; Bensch, Staffan LU ; Laybourn-Parry, Johanna and Rengefors, Karin LU (2009) In Protist 160. p.301-317
Abstract
Little is known about the extent of the genetic diversity and its structuring patterns in protist species living in lakes. Here, we have investigated the genetic diversity patterns within five dinoflagellate species (Peridinium aciculiferum, Peridinium cinctum, Peridiniopsis borgei, Polarella glacialis, Scrippsiella aff. hangoei) that are present in lakes and sometimes, in marine habitats located in polar and temperate regions. A total of 68 clonal strains were investigated using Amplified Fragment Length Polymorphism (AFLP), a sensitive genetic fingerprinting technique. All used strains within each species had identical ITS nuclear ribosomal DNA sequences, a characteristic that indicates that they likely belong to the same species. We... (More)
Little is known about the extent of the genetic diversity and its structuring patterns in protist species living in lakes. Here, we have investigated the genetic diversity patterns within five dinoflagellate species (Peridinium aciculiferum, Peridinium cinctum, Peridiniopsis borgei, Polarella glacialis, Scrippsiella aff. hangoei) that are present in lakes and sometimes, in marine habitats located in polar and temperate regions. A total of 68 clonal strains were investigated using Amplified Fragment Length Polymorphism (AFLP), a sensitive genetic fingerprinting technique. All used strains within each species had identical ITS nuclear ribosomal DNA sequences, a characteristic that indicates that they likely belong to the same species. We found a wide variability in the genetic diversity among species (between 20% and 90% of polymorphic loci; Nei's gene diversity between 0.08 and 0.37). In some cases, our analyses suggested the presence of different genetically homogeneous subgroups (genetic populations) within the same water body. Thus, it appears that different genetic populations can coexist within the same lake despite the likely occurrence of recombination that tends to homogenize the gene pool. Overall, our results indicated that a large number of dinoflagellate genotypes are present in lake populations, instead of a few dominating ones. In addition, our study shows that protists with identical ITS sequences can harbor considerable amounts of genetic diversity. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Protist
volume
160
pages
301 - 317
publisher
Elsevier
external identifiers
  • wos:000265079100010
  • scopus:64449087764
ISSN
1434-4610
DOI
10.1016/j.protis.2008.10.004
language
English
LU publication?
yes
id
0fecc4c2-f459-4802-a12a-70376d5fcc98 (old id 1289362)
date added to LUP
2009-02-16 13:25:00
date last changed
2017-04-23 03:32:24
@article{0fecc4c2-f459-4802-a12a-70376d5fcc98,
  abstract     = {Little is known about the extent of the genetic diversity and its structuring patterns in protist species living in lakes. Here, we have investigated the genetic diversity patterns within five dinoflagellate species (Peridinium aciculiferum, Peridinium cinctum, Peridiniopsis borgei, Polarella glacialis, Scrippsiella aff. hangoei) that are present in lakes and sometimes, in marine habitats located in polar and temperate regions. A total of 68 clonal strains were investigated using Amplified Fragment Length Polymorphism (AFLP), a sensitive genetic fingerprinting technique. All used strains within each species had identical ITS nuclear ribosomal DNA sequences, a characteristic that indicates that they likely belong to the same species. We found a wide variability in the genetic diversity among species (between 20% and 90% of polymorphic loci; Nei's gene diversity between 0.08 and 0.37). In some cases, our analyses suggested the presence of different genetically homogeneous subgroups (genetic populations) within the same water body. Thus, it appears that different genetic populations can coexist within the same lake despite the likely occurrence of recombination that tends to homogenize the gene pool. Overall, our results indicated that a large number of dinoflagellate genotypes are present in lake populations, instead of a few dominating ones. In addition, our study shows that protists with identical ITS sequences can harbor considerable amounts of genetic diversity.},
  author       = {Logares, Ramiro and Boltovskoy, Andrés and Bensch, Staffan and Laybourn-Parry, Johanna and Rengefors, Karin},
  issn         = {1434-4610},
  language     = {eng},
  pages        = {301--317},
  publisher    = {Elsevier},
  series       = {Protist},
  title        = {Genetic Diversity Patterns in Five Protist Species Occurring in Lakes.},
  url          = {http://dx.doi.org/10.1016/j.protis.2008.10.004},
  volume       = {160},
  year         = {2009},
}