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Improved selectivity of microbial biosensor using membrane coating. Application to the analysis of ethanol during fermentation

Tkac, Jan LU ; Vostiar, Igor LU ; Gorton, Lo LU ; Gemeiner, P and Sturdik, E (2003) In Biosensors & Bioelectronics 18(9). p.1125-1134
Abstract
A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 M (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors... (More)
A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 M (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l−1 (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors & Bioelectronics
volume
18
issue
9
pages
1125 - 1134
publisher
Elsevier
external identifiers
  • wos:000183942800006
  • pmid:12788555
  • scopus:0038360817
ISSN
1873-4235
DOI
10.1016/S0956-5663(02)00244-0
language
English
LU publication?
yes
id
bf1454a3-2e2b-4566-a2d3-85f8ef23d18c (old id 128999)
date added to LUP
2007-06-28 09:04:26
date last changed
2018-02-18 04:31:53
@article{bf1454a3-2e2b-4566-a2d3-85f8ef23d18c,
  abstract     = {A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 M (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l−1},
  author       = {Tkac, Jan and Vostiar, Igor and Gorton, Lo and Gemeiner, P and Sturdik, E},
  issn         = {1873-4235},
  language     = {eng},
  number       = {9},
  pages        = {1125--1134},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Improved selectivity of microbial biosensor using membrane coating. Application to the analysis of ethanol during fermentation},
  url          = {http://dx.doi.org/10.1016/S0956-5663(02)00244-0},
  volume       = {18},
  year         = {2003},
}