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Incomplete degradation of polycyclic aromatic hydrocarbons in soil inoculated with wood rotting fungi and their effect on the indigenous soil bacteria.

Andersson, Erik LU ; Lundstedt, S; Tornberg, Karin LU ; Schnürer, Y; Öberg, L and Mattiasson, Bo LU (2003) In Rangeland Ecology & Management 22(6). p.1238-1243
Abstract
Soil artificially contaminated with fluorene, phenanthrene, pyrene, and benz[a]anthracene was inoculated with the wood-rotting fungi Pleurotus ostreatus and Antrodia vaillantii. During 12 weeks of incubation, polycyclic aromatic hydrocarbon (PAH) degradation and the formation of persistent degradation products were monitored by chemical analysis. In addition, the effect on the indigenous soil bacteria was studied by plate count techniques and by measuring the concentration of bacteria-specific phospholipid fatty acids (PLFAs). In both soils inoculated with fungi, the PAH degradation was enhanced compared to the control soil without fungi. The white-rot fungus P. ostreatus accelerated the degradation rate radically the first weeks, while... (More)
Soil artificially contaminated with fluorene, phenanthrene, pyrene, and benz[a]anthracene was inoculated with the wood-rotting fungi Pleurotus ostreatus and Antrodia vaillantii. During 12 weeks of incubation, polycyclic aromatic hydrocarbon (PAH) degradation and the formation of persistent degradation products were monitored by chemical analysis. In addition, the effect on the indigenous soil bacteria was studied by plate count techniques and by measuring the concentration of bacteria-specific phospholipid fatty acids (PLFAs). In both soils inoculated with fungi, the PAH degradation was enhanced compared to the control soil without fungi. The white-rot fungus P. ostreatus accelerated the degradation rate radically the first weeks, while the effect of the brown-rot fungus was more pronounced at later stages during the 12-week study. In a soil with no amendments, the final degradation result was similar to that in the soil with added fungi, although the degradation pattern for the individual PAHs was different. Furthermore, the degradation by P. ostreatus was accompanied by an accumulation of PAH metabolites, that is, 9-fluorenone, benz[a]anthracene-7,12-dione, and two compounds identified as 4-hydroxy-9-fluorenone and 4-oxapyrene-5-one, that was not seen in the other soils. The inoculation with the white-rot fungus also had a large negative effect on the indigenous soil bacteria. This could be an important drawback of using the white-rot fungus P. ostreatus in soil bioremediation since a sequential fungal–bacterial degradation probably is needed for a complete degradation of PAHs in soil. In the soil inoculated with A. vaillantii, on the other hand, no metabolites accumulated, and no negative effects were observed on the indigenous microorganisms. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
soil white-rot fungi, hydrocarbon metabolites, polycyclic aromatic, polycyclic aromatic hydrocarbons, degradation
in
Rangeland Ecology & Management
volume
22
issue
6
pages
1238 - 1243
publisher
Society of Environmental Toxicology and Chemistry
external identifiers
  • pmid:12785579
  • wos:000185639100008
  • scopus:0012938661
ISSN
1550-7424
DOI
10.1002/etc.5620220608
language
English
LU publication?
yes
id
86595ddb-5232-4ff6-8c9a-2487b18401df (old id 129043)
date added to LUP
2007-06-29 15:48:15
date last changed
2018-06-24 03:49:32
@article{86595ddb-5232-4ff6-8c9a-2487b18401df,
  abstract     = {Soil artificially contaminated with fluorene, phenanthrene, pyrene, and benz[a]anthracene was inoculated with the wood-rotting fungi Pleurotus ostreatus and Antrodia vaillantii. During 12 weeks of incubation, polycyclic aromatic hydrocarbon (PAH) degradation and the formation of persistent degradation products were monitored by chemical analysis. In addition, the effect on the indigenous soil bacteria was studied by plate count techniques and by measuring the concentration of bacteria-specific phospholipid fatty acids (PLFAs). In both soils inoculated with fungi, the PAH degradation was enhanced compared to the control soil without fungi. The white-rot fungus P. ostreatus accelerated the degradation rate radically the first weeks, while the effect of the brown-rot fungus was more pronounced at later stages during the 12-week study. In a soil with no amendments, the final degradation result was similar to that in the soil with added fungi, although the degradation pattern for the individual PAHs was different. Furthermore, the degradation by P. ostreatus was accompanied by an accumulation of PAH metabolites, that is, 9-fluorenone, benz[a]anthracene-7,12-dione, and two compounds identified as 4-hydroxy-9-fluorenone and 4-oxapyrene-5-one, that was not seen in the other soils. The inoculation with the white-rot fungus also had a large negative effect on the indigenous soil bacteria. This could be an important drawback of using the white-rot fungus P. ostreatus in soil bioremediation since a sequential fungal–bacterial degradation probably is needed for a complete degradation of PAHs in soil. In the soil inoculated with A. vaillantii, on the other hand, no metabolites accumulated, and no negative effects were observed on the indigenous microorganisms.},
  author       = {Andersson, Erik and Lundstedt, S and Tornberg, Karin and Schnürer, Y and Öberg, L and Mattiasson, Bo},
  issn         = {1550-7424},
  keyword      = {soil white-rot fungi,hydrocarbon metabolites,polycyclic aromatic,polycyclic aromatic hydrocarbons,degradation},
  language     = {eng},
  number       = {6},
  pages        = {1238--1243},
  publisher    = {Society of Environmental Toxicology and Chemistry},
  series       = {Rangeland Ecology & Management},
  title        = {Incomplete degradation of polycyclic aromatic hydrocarbons in soil inoculated with wood rotting fungi and their effect on the indigenous soil bacteria.},
  url          = {http://dx.doi.org/10.1002/etc.5620220608},
  volume       = {22},
  year         = {2003},
}