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Cold adapted yeasts as producers of cold active polygalacturonases

Birgisson, Hakon LU ; Delgado, Osvaldo LU ; Carcia Arroyo, L; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU (2003) In Extremophiles 7(3). p.185-193
Abstract
Eight cold-adapted, polygalacturonase-producing yeasts belonging to four species were isolated from frozen environmental samples in Iceland. They were identified as Cystofilobasidium lari-marini, Cystofilobasidium capitatum, Cryptococcus macerans and Cryptococcus aquaticus species by sequence analysis of rDNA regions. Growth behavior of the isolates was investigated. All strains could grow at 2°C. Addition of glucose to pectin-containing culture medium had a repressive effect on enzyme production except for C. aquaticus, which showed increased polygalacturonase activity. Optimal temperature for enzyme production for the Cystofilobasidium strains was 14°C, while that for the Cryptococcus strains was lower. Among the isolates, C. lari-marini... (More)
Eight cold-adapted, polygalacturonase-producing yeasts belonging to four species were isolated from frozen environmental samples in Iceland. They were identified as Cystofilobasidium lari-marini, Cystofilobasidium capitatum, Cryptococcus macerans and Cryptococcus aquaticus species by sequence analysis of rDNA regions. Growth behavior of the isolates was investigated. All strains could grow at 2°C. Addition of glucose to pectin-containing culture medium had a repressive effect on enzyme production except for C. aquaticus, which showed increased polygalacturonase activity. Optimal temperature for enzyme production for the Cystofilobasidium strains was 14°C, while that for the Cryptococcus strains was lower. Among the isolates, C. lari-marini S3B produced highest levels of enzyme activity at pH 3.2. Preliminary characterization of the polygalacturonases in the culture supernatant showed the enzyme from Cystofilobasidium strains to be optimally active at 40°C and pH 5, and that from the Cryptococcus strains at 50°C and pH 4. The polygalacturonase from C. macerans started to lose activity after 1 h of incubation at 40°C, while that from the other strains had already lost activity at 30°C. All the strains except C. aquaticus produced isoenzymes of polyglacturonase. In addition to polygalacturonase, the Cystofilobasidium strains produced pectin lyase, C. aquaticus pectin esterase, and C. macerans pectin lyase, pectate lyase and pectin esterase. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Extremophiles
volume
7
issue
3
pages
185 - 193
publisher
Springer
external identifiers
  • wos:000183800200003
  • pmid:12768449
  • scopus:0345671157
ISSN
1433-4909
DOI
10.1007/s00792-002-0310-7
language
English
LU publication?
yes
id
6d829088-9f84-4404-9006-6d683383b92b (old id 129091)
date added to LUP
2007-07-02 08:19:35
date last changed
2018-06-17 03:50:10
@article{6d829088-9f84-4404-9006-6d683383b92b,
  abstract     = {Eight cold-adapted, polygalacturonase-producing yeasts belonging to four species were isolated from frozen environmental samples in Iceland. They were identified as Cystofilobasidium lari-marini, Cystofilobasidium capitatum, Cryptococcus macerans and Cryptococcus aquaticus species by sequence analysis of rDNA regions. Growth behavior of the isolates was investigated. All strains could grow at 2°C. Addition of glucose to pectin-containing culture medium had a repressive effect on enzyme production except for C. aquaticus, which showed increased polygalacturonase activity. Optimal temperature for enzyme production for the Cystofilobasidium strains was 14°C, while that for the Cryptococcus strains was lower. Among the isolates, C. lari-marini S3B produced highest levels of enzyme activity at pH 3.2. Preliminary characterization of the polygalacturonases in the culture supernatant showed the enzyme from Cystofilobasidium strains to be optimally active at 40°C and pH 5, and that from the Cryptococcus strains at 50°C and pH 4. The polygalacturonase from C. macerans started to lose activity after 1 h of incubation at 40°C, while that from the other strains had already lost activity at 30°C. All the strains except C. aquaticus produced isoenzymes of polyglacturonase. In addition to polygalacturonase, the Cystofilobasidium strains produced pectin lyase, C. aquaticus pectin esterase, and C. macerans pectin lyase, pectate lyase and pectin esterase.},
  author       = {Birgisson, Hakon and Delgado, Osvaldo and Carcia Arroyo, L and Hatti-Kaul, Rajni and Mattiasson, Bo},
  issn         = {1433-4909},
  language     = {eng},
  number       = {3},
  pages        = {185--193},
  publisher    = {Springer},
  series       = {Extremophiles},
  title        = {Cold adapted yeasts as producers of cold active polygalacturonases},
  url          = {http://dx.doi.org/10.1007/s00792-002-0310-7},
  volume       = {7},
  year         = {2003},
}