Advanced

Beta-estradiol effect on erythrocyte aggregation--a controlled in vitro study

Goncalves, Isabel LU ; Saldanha, C and Martins e Silva, J (2001) In Clinical Hemorheology and Microcirculation 25(3-4). p.127-134
Abstract
The purpose of this in vitro study was to assess the effect of 17beta-estradiol on hemorheologic parameters, namely on erythrocyte aggregation and deformability and membrane fluidity.Blood samples from 65 women (aged 57 +/- 4 years) undergoing postmenopausal hormone replacement therapy were obtained and were incubated for 5 min in absence and presence of 17beta-estradiol 10(-5) M. The measured parameters were the erythrocyte aggregation (EAI) and deformability (EI), the acetylcholinesterase activity (AChE), the plasma pH and osmolality and the erythrocyte membrane fluidity assessed by fluorescence polarization with two probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and (1-(4-(trimethylamino)-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). Data... (More)
The purpose of this in vitro study was to assess the effect of 17beta-estradiol on hemorheologic parameters, namely on erythrocyte aggregation and deformability and membrane fluidity.Blood samples from 65 women (aged 57 +/- 4 years) undergoing postmenopausal hormone replacement therapy were obtained and were incubated for 5 min in absence and presence of 17beta-estradiol 10(-5) M. The measured parameters were the erythrocyte aggregation (EAI) and deformability (EI), the acetylcholinesterase activity (AChE), the plasma pH and osmolality and the erythrocyte membrane fluidity assessed by fluorescence polarization with two probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and (1-(4-(trimethylamino)-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). Data analysis was performed using t-Student and Pearson correlation analysis. A statistically significant decrease of the EAI (15.8 +/- 3.02 vs 13.45 +/- 2.3; p<0.001) and an increase of the EI (51.39 +/- 5.64 vs 52.06 +/- 5.36; p<0.01) at shear stress of 30 Pa in presence of 17beta-estradiol 10(-5) M was obtained. There was a decrease in membrane fluidity in 45 blood samples (DPH) and in the other 20 an increase, when beta-estradiol 10(-5) M was present. In vitro beta-estradiol 10(-5) M decreased erythrocyte aggregation in blood samples of postmenopausal women undergoing hormone therapy, which could prevent high blood viscosity and, consequently, cardiovascular events. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Hemorheology and Microcirculation
volume
25
issue
3-4
pages
127 - 134
publisher
IOS Press
external identifiers
  • wos:000174153500005
ISSN
1386-0291
language
English
LU publication?
no
id
34d4fa20-291f-4f31-a3c8-547eaa13a56e (old id 1297340)
date added to LUP
2009-07-14 14:01:24
date last changed
2018-05-29 10:52:22
@article{34d4fa20-291f-4f31-a3c8-547eaa13a56e,
  abstract     = {The purpose of this in vitro study was to assess the effect of 17beta-estradiol on hemorheologic parameters, namely on erythrocyte aggregation and deformability and membrane fluidity.Blood samples from 65 women (aged 57 +/- 4 years) undergoing postmenopausal hormone replacement therapy were obtained and were incubated for 5 min in absence and presence of 17beta-estradiol 10(-5) M. The measured parameters were the erythrocyte aggregation (EAI) and deformability (EI), the acetylcholinesterase activity (AChE), the plasma pH and osmolality and the erythrocyte membrane fluidity assessed by fluorescence polarization with two probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and (1-(4-(trimethylamino)-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). Data analysis was performed using t-Student and Pearson correlation analysis. A statistically significant decrease of the EAI (15.8 +/- 3.02 vs 13.45 +/- 2.3; p&lt;0.001) and an increase of the EI (51.39 +/- 5.64 vs 52.06 +/- 5.36; p&lt;0.01) at shear stress of 30 Pa in presence of 17beta-estradiol 10(-5) M was obtained. There was a decrease in membrane fluidity in 45 blood samples (DPH) and in the other 20 an increase, when beta-estradiol 10(-5) M was present. In vitro beta-estradiol 10(-5) M decreased erythrocyte aggregation in blood samples of postmenopausal women undergoing hormone therapy, which could prevent high blood viscosity and, consequently, cardiovascular events.},
  author       = {Goncalves, Isabel and Saldanha, C and Martins e Silva, J},
  issn         = {1386-0291},
  language     = {eng},
  number       = {3-4},
  pages        = {127--134},
  publisher    = {IOS Press},
  series       = {Clinical Hemorheology and Microcirculation},
  title        = {Beta-estradiol effect on erythrocyte aggregation--a controlled in vitro study},
  volume       = {25},
  year         = {2001},
}