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In vivo and in vitro effects of flutamide and diethylstilbestrol on fetal testicular steroidogenesis in the rat

Adamsson, N A; Brokken, Leon LU ; Paranko, J and Toppari, J (2008) In Reproductive Toxicology 25(1). p.76-83
Abstract
Prenatal testosterone surge is considered crucial for physiological masculinization of male progeny. Disorders in sex steroid hormone balance during the fetal development may interfere with male reproductive health later in life. In this study, we have investigated in utero and in vitro effects of flutamide (FLU) and diethylstilbestrol (DES) on fetal rat testicular steroidogenesis. In utero exposure to FLU 25mg/kg or DES 0.02mg/kg had no obvious effects on ED 19.5 rat testicular testosterone and progesterone production, StAR protein or AR protein expression. However, when ED 19.5 rat testis were cultured for 180min in the presence of 0.1, 1, 10 and 100mg/l of FLU or DES, the highest doses of both compounds were capable of disturbing... (More)
Prenatal testosterone surge is considered crucial for physiological masculinization of male progeny. Disorders in sex steroid hormone balance during the fetal development may interfere with male reproductive health later in life. In this study, we have investigated in utero and in vitro effects of flutamide (FLU) and diethylstilbestrol (DES) on fetal rat testicular steroidogenesis. In utero exposure to FLU 25mg/kg or DES 0.02mg/kg had no obvious effects on ED 19.5 rat testicular testosterone and progesterone production, StAR protein or AR protein expression. However, when ED 19.5 rat testis were cultured for 180min in the presence of 0.1, 1, 10 and 100mg/l of FLU or DES, the highest doses of both compounds were capable of disturbing steroidogenesis. To study the rate of the changes seen in testicular steroidogenesis after 180min, time-series experiments, in which intact testes were cultured with FLU 100mg/l or DES 100mg/l for 30, 60 or 120min, were performed. In vitro time-series experiments revealed that changes in steroidogenesis occur very fast. Experiments with FLU brought further evidence to the hypothesis that ARs have negative autocrine role in developing Leydig cells. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Reproductive Toxicology
volume
25
issue
1
pages
76 - 83
publisher
Elsevier
external identifiers
  • wos:000253097900009
  • scopus:37349049958
ISSN
1873-1708
DOI
10.1016/j.reprotox.2007.08.001
language
English
LU publication?
no
id
97b1a861-befa-4ec2-a543-c2a523e79d1d (old id 1298581)
date added to LUP
2009-07-10 11:01:20
date last changed
2017-01-01 05:31:03
@article{97b1a861-befa-4ec2-a543-c2a523e79d1d,
  abstract     = {Prenatal testosterone surge is considered crucial for physiological masculinization of male progeny. Disorders in sex steroid hormone balance during the fetal development may interfere with male reproductive health later in life. In this study, we have investigated in utero and in vitro effects of flutamide (FLU) and diethylstilbestrol (DES) on fetal rat testicular steroidogenesis. In utero exposure to FLU 25mg/kg or DES 0.02mg/kg had no obvious effects on ED 19.5 rat testicular testosterone and progesterone production, StAR protein or AR protein expression. However, when ED 19.5 rat testis were cultured for 180min in the presence of 0.1, 1, 10 and 100mg/l of FLU or DES, the highest doses of both compounds were capable of disturbing steroidogenesis. To study the rate of the changes seen in testicular steroidogenesis after 180min, time-series experiments, in which intact testes were cultured with FLU 100mg/l or DES 100mg/l for 30, 60 or 120min, were performed. In vitro time-series experiments revealed that changes in steroidogenesis occur very fast. Experiments with FLU brought further evidence to the hypothesis that ARs have negative autocrine role in developing Leydig cells.},
  author       = {Adamsson, N A and Brokken, Leon and Paranko, J and Toppari, J},
  issn         = {1873-1708},
  language     = {eng},
  number       = {1},
  pages        = {76--83},
  publisher    = {Elsevier},
  series       = {Reproductive Toxicology},
  title        = {In vivo and in vitro effects of flutamide and diethylstilbestrol on fetal testicular steroidogenesis in the rat},
  url          = {http://dx.doi.org/10.1016/j.reprotox.2007.08.001},
  volume       = {25},
  year         = {2008},
}