Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.
(2007) In Neuroscience Letters 428(1). p.47-51- Abstract
- Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100%... (More)
- Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1298974
- author
- Severino, Maurizio LU ; Pedersen, AF ; Trajkovska, V ; Christensen, E ; Lohals, R ; Veng, LM ; Knudsen, GM and Aznar, S
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Neuroscience Letters
- volume
- 428
- issue
- 1
- pages
- 47 - 51
- publisher
- Elsevier
- external identifiers
-
- scopus:35448937166
- ISSN
- 0304-3940
- DOI
- 10.1016/j.neulet.2007.09.026
- language
- English
- LU publication?
- no
- id
- b56423ec-c87e-4e64-852c-5bde9806c212 (old id 1298974)
- date added to LUP
- 2016-04-04 10:11:37
- date last changed
- 2022-01-29 19:53:46
@article{b56423ec-c87e-4e64-852c-5bde9806c212, abstract = {{Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca}}, author = {{Severino, Maurizio and Pedersen, AF and Trajkovska, V and Christensen, E and Lohals, R and Veng, LM and Knudsen, GM and Aznar, S}}, issn = {{0304-3940}}, language = {{eng}}, number = {{1}}, pages = {{47--51}}, publisher = {{Elsevier}}, series = {{Neuroscience Letters}}, title = {{Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.}}, url = {{http://dx.doi.org/10.1016/j.neulet.2007.09.026}}, doi = {{10.1016/j.neulet.2007.09.026}}, volume = {{428}}, year = {{2007}}, }