Defining immunological impact and therapeutic benefit of mild heating in a murine model of arthritis
Lee, Chen-Ting ; Kokolus, Kathleen M ; Leigh, Nicholas D LU
; Capitano, Maegan
; Hylander, Bonnie L
and Repasky, Elizabeth A
(2015)
In PLoS ONE
10(3).
p.0120327-0120327
- Abstract
Traditional treatments, including a variety of thermal therapies have been known since ancient times to provide relief from rheumatoid arthritis (RA) symptoms. However, a general absence of information on how heating affects molecular or immunological targets relevant to RA has limited heat treatment (HT) to the category of treatments known as "alternative therapies". In this study, we evaluated the effectiveness of mild HT in a collagen-induced arthritis (CIA) model which has been used in many previous studies to evaluate newer pharmacological approaches for the treatment of RA, and tested whether inflammatory immune activity was altered. We also compared the effect of HT to methotrexate, a well characterized pharmacological treatment... (More)
Traditional treatments, including a variety of thermal therapies have been known since ancient times to provide relief from rheumatoid arthritis (RA) symptoms. However, a general absence of information on how heating affects molecular or immunological targets relevant to RA has limited heat treatment (HT) to the category of treatments known as "alternative therapies". In this study, we evaluated the effectiveness of mild HT in a collagen-induced arthritis (CIA) model which has been used in many previous studies to evaluate newer pharmacological approaches for the treatment of RA, and tested whether inflammatory immune activity was altered. We also compared the effect of HT to methotrexate, a well characterized pharmacological treatment for RA. CIA mice were treated with either a single HT for several hours or daily 30 minute HT. Disease progression and macrophage infiltration were evaluated. We found that both HT regimens significantly reduced arthritis disease severity and macrophage infiltration into inflamed joints. Surprisingly, HT was as efficient as methotrexate in controlling disease progression. At the molecular level, HT suppressed TNF-α while increasing production of IL-10. We also observed an induction of HSP70 and a reduction in both NF-κB and HIF-1α in inflamed tissues. Additionally, using activated macrophages in vitro, we found that HT reduced production of pro-inflammatory cytokines, an effect which is correlated to induction of HSF-1 and HSP70 and inhibition of NF-κB and STAT activation. Our findings demonstrate a significant therapeutic benefit of HT in controlling arthritis progression in a clinically relevant mouse model, with an efficacy similar to methotrexate. Mechanistically, HT targets highly relevant anti-inflammatory pathways which strongly support its increased study for use in clinical trials for RA.
(Less)
- author
- Lee, Chen-Ting
; Kokolus, Kathleen M
; Leigh, Nicholas D
LU
; Capitano, Maegan
; Hylander, Bonnie L
and Repasky, Elizabeth A
- publishing date
- 2015-03-20
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Antibodies/immunology, Antirheumatic Agents/pharmacology, Arthritis, Experimental/immunology, Arthritis, Rheumatoid/immunology, Combined Modality Therapy, Cytokines/metabolism, Disease Models, Animal, Heat-Shock Proteins/genetics, Hyperthermia, Induced, Joints/drug effects, Macrophage Activation/immunology, Macrophages/immunology, Methotrexate/pharmacology, Mice, NF-kappa B/metabolism, STAT1 Transcription Factor/metabolism, T-Lymphocytes/immunology
- in
- PLoS ONE
- volume
- 10
- issue
- 3
- pages
- 0120327 - 0120327
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:25793532
- scopus:84925799562
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0120327
- language
- English
- LU publication?
- no
- id
- 12f19362-7bf8-4f57-b31e-d64b5c2cba15
- date added to LUP
- 2020-04-30 23:19:02
- date last changed
- 2024-01-31 20:31:47
@article{12f19362-7bf8-4f57-b31e-d64b5c2cba15,
abstract = {{<p>Traditional treatments, including a variety of thermal therapies have been known since ancient times to provide relief from rheumatoid arthritis (RA) symptoms. However, a general absence of information on how heating affects molecular or immunological targets relevant to RA has limited heat treatment (HT) to the category of treatments known as "alternative therapies". In this study, we evaluated the effectiveness of mild HT in a collagen-induced arthritis (CIA) model which has been used in many previous studies to evaluate newer pharmacological approaches for the treatment of RA, and tested whether inflammatory immune activity was altered. We also compared the effect of HT to methotrexate, a well characterized pharmacological treatment for RA. CIA mice were treated with either a single HT for several hours or daily 30 minute HT. Disease progression and macrophage infiltration were evaluated. We found that both HT regimens significantly reduced arthritis disease severity and macrophage infiltration into inflamed joints. Surprisingly, HT was as efficient as methotrexate in controlling disease progression. At the molecular level, HT suppressed TNF-α while increasing production of IL-10. We also observed an induction of HSP70 and a reduction in both NF-κB and HIF-1α in inflamed tissues. Additionally, using activated macrophages in vitro, we found that HT reduced production of pro-inflammatory cytokines, an effect which is correlated to induction of HSF-1 and HSP70 and inhibition of NF-κB and STAT activation. Our findings demonstrate a significant therapeutic benefit of HT in controlling arthritis progression in a clinically relevant mouse model, with an efficacy similar to methotrexate. Mechanistically, HT targets highly relevant anti-inflammatory pathways which strongly support its increased study for use in clinical trials for RA. </p>}},
author = {{Lee, Chen-Ting and Kokolus, Kathleen M and Leigh, Nicholas D and Capitano, Maegan and Hylander, Bonnie L and Repasky, Elizabeth A}},
issn = {{1932-6203}},
keywords = {{Animals; Antibodies/immunology; Antirheumatic Agents/pharmacology; Arthritis, Experimental/immunology; Arthritis, Rheumatoid/immunology; Combined Modality Therapy; Cytokines/metabolism; Disease Models, Animal; Heat-Shock Proteins/genetics; Hyperthermia, Induced; Joints/drug effects; Macrophage Activation/immunology; Macrophages/immunology; Methotrexate/pharmacology; Mice; NF-kappa B/metabolism; STAT1 Transcription Factor/metabolism; T-Lymphocytes/immunology}},
language = {{eng}},
month = {{03}},
number = {{3}},
pages = {{0120327--0120327}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{Defining immunological impact and therapeutic benefit of mild heating in a murine model of arthritis}},
url = {{https://lup.lub.lu.se/search/files/79042613/2015_Lee_PLoS.PDF}},
doi = {{10.1371/journal.pone.0120327}},
volume = {{10}},
year = {{2015}},
}