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Bidirectionality and transcriptional activity of the EWSR1 promoter region

Möller, Emely LU ; Mandahl, Nils LU ; Iliszko, Mariola; Mertens, Fredrik LU and Panagopoulos, Ioannis LU (2009) In Oncology Reports 21(3). p.641-648
Abstract

EWSR1 is involved in chimeric proteins which play crucial roles in the development of a variety of bone and soft tissue tumors. Many of the chimeric genes involving EWSR1 have been extensively studied, whereas less is known about the wild-type (wt) gene and its regulation. As the expression of the chimeric gene is driven by the EWSR1 promoter, it is of importance to study the mechanisms regulating wt EWSR1 expression. We estimated the transcriptional activity of the EWSR1 promoter through deletion fragments driving reporter gene expression. This assay identified the 100-bp region immediately downstream of the EWSR1 transcriptional start site (+1) and the downstream region from +100 to +300 as important regions for transcriptional... (More)

EWSR1 is involved in chimeric proteins which play crucial roles in the development of a variety of bone and soft tissue tumors. Many of the chimeric genes involving EWSR1 have been extensively studied, whereas less is known about the wild-type (wt) gene and its regulation. As the expression of the chimeric gene is driven by the EWSR1 promoter, it is of importance to study the mechanisms regulating wt EWSR1 expression. We estimated the transcriptional activity of the EWSR1 promoter through deletion fragments driving reporter gene expression. This assay identified the 100-bp region immediately downstream of the EWSR1 transcriptional start site (+1) and the downstream region from +100 to +300 as important regions for transcriptional regulation. We also found that EWSR1 and RHBDD3, a gene located directly upstream of EWSR1 that is likely to share regulatory elements with EWSR1, were co-expressed in the tissue panels, Ewing tumor biopsies and cell lines. Thus, our results show that the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3, and identifies specific regions that strongly influence promoter activity.

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organization
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Contribution to journal
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published
subject
keywords
Animals, Apoptosis Regulatory Proteins, Base Sequence, Bone Neoplasms, Calmodulin-Binding Proteins, Conserved Sequence, Gene Expression Regulation, Humans, Molecular Sequence Data, Neoplasm Proteins, Promoter Regions, Genetic, RNA-Binding Proteins, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma, Ewing, Sequence Homology, Nucleic Acid, Transcription, Genetic, Transfection, Journal Article, Research Support, Non-U.S. Gov't
in
Oncology Reports
volume
21
issue
3
pages
8 pages
publisher
D.A. Spandidos
external identifiers
  • wos:000263584000010
  • pmid:19212622
  • scopus:64849117176
ISSN
1791-2431
DOI
10.3892/or_00000267
language
English
LU publication?
yes
id
9e540e0b-7b27-484c-82f8-b71ba58c68d5 (old id 1302646)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19212622?dopt=Abstract
date added to LUP
2009-03-02 14:23:02
date last changed
2017-01-03 09:43:27
@article{9e540e0b-7b27-484c-82f8-b71ba58c68d5,
  abstract     = {<p>EWSR1 is involved in chimeric proteins which play crucial roles in the development of a variety of bone and soft tissue tumors. Many of the chimeric genes involving EWSR1 have been extensively studied, whereas less is known about the wild-type (wt) gene and its regulation. As the expression of the chimeric gene is driven by the EWSR1 promoter, it is of importance to study the mechanisms regulating wt EWSR1 expression. We estimated the transcriptional activity of the EWSR1 promoter through deletion fragments driving reporter gene expression. This assay identified the 100-bp region immediately downstream of the EWSR1 transcriptional start site (+1) and the downstream region from +100 to +300 as important regions for transcriptional regulation. We also found that EWSR1 and RHBDD3, a gene located directly upstream of EWSR1 that is likely to share regulatory elements with EWSR1, were co-expressed in the tissue panels, Ewing tumor biopsies and cell lines. Thus, our results show that the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3, and identifies specific regions that strongly influence promoter activity.</p>},
  author       = {Möller, Emely and Mandahl, Nils and Iliszko, Mariola and Mertens, Fredrik and Panagopoulos, Ioannis},
  issn         = {1791-2431},
  keyword      = {Animals,Apoptosis Regulatory Proteins,Base Sequence,Bone Neoplasms,Calmodulin-Binding Proteins,Conserved Sequence,Gene Expression Regulation,Humans,Molecular Sequence Data,Neoplasm Proteins,Promoter Regions, Genetic,RNA-Binding Proteins,Reverse Transcriptase Polymerase Chain Reaction,Sarcoma, Ewing,Sequence Homology, Nucleic Acid,Transcription, Genetic,Transfection,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {641--648},
  publisher    = {D.A. Spandidos},
  series       = {Oncology Reports},
  title        = {Bidirectionality and transcriptional activity of the EWSR1 promoter region},
  url          = {http://dx.doi.org/10.3892/or_00000267},
  volume       = {21},
  year         = {2009},
}