Characterization of the human CREB3L2 gene promoter
(2009) In Oncology Reports 21(3). p.615-624- Abstract
CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol... (More)
CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.
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- author
- Panagopoulos, Ioannis LU and Mertens, Fredrik LU
- organization
- publishing date
- 2009-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Base Sequence, Basic-Leucine Zipper Transcription Factors, Gene Expression Regulation, Humans, Molecular Sequence Data, Mutagenesis, Site-Directed, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Journal Article, Research Support, Non-U.S. Gov't
- in
- Oncology Reports
- volume
- 21
- issue
- 3
- pages
- 10 pages
- publisher
- Spandidos Publications
- external identifiers
-
- wos:000263584000007
- pmid:19212619
- scopus:64849107204
- pmid:19212619
- ISSN
- 1791-2431
- DOI
- 10.3892/or_00000264
- language
- English
- LU publication?
- yes
- id
- 946ee665-f664-4c15-b18f-83ebf6766f78 (old id 1302653)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19212619?dopt=Abstract
- date added to LUP
- 2016-04-04 09:04:41
- date last changed
- 2022-02-28 06:16:13
@article{946ee665-f664-4c15-b18f-83ebf6766f78, abstract = {{<p>CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.</p>}}, author = {{Panagopoulos, Ioannis and Mertens, Fredrik}}, issn = {{1791-2431}}, keywords = {{Base Sequence; Basic-Leucine Zipper Transcription Factors; Gene Expression Regulation; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Nucleic Acid; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, number = {{3}}, pages = {{615--624}}, publisher = {{Spandidos Publications}}, series = {{Oncology Reports}}, title = {{Characterization of the human CREB3L2 gene promoter}}, url = {{http://dx.doi.org/10.3892/or_00000264}}, doi = {{10.3892/or_00000264}}, volume = {{21}}, year = {{2009}}, }