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Formation of new chromosomes as a virulence mechanism in yeast Candida glabrata.

Poláková, Silvia LU ; Blume, Christian; Zárate, Julián Alvarez; Mentel, Marek; Jørck-Ramberg, Dorte; Stenderup, Jørgen and Piskur, Jure LU (2009) In Proceedings of the National Academy of Sciences 106(8). p.2688-2693
Abstract
In eukaryotes, the number and rough organization of chromosomes is well preserved within isolates of the same species. Novel chromosomes and loss of chromosomes are infrequent and usually associated with pathological events. Here, we analyzed 40 pathogenic isolates of a haploid and asexual yeast, Candida glabrata, for their genome structure and stability. This organism has recently become the second most prevalent yeast pathogen in humans. Although the gene sequences were well conserved among different strains, their chromosome structures differed drastically. The most frequent events reshaping chromosomes were translocations of chromosomal arms. However, also larger segmental duplications were frequent and occasionally we observed novel... (More)
In eukaryotes, the number and rough organization of chromosomes is well preserved within isolates of the same species. Novel chromosomes and loss of chromosomes are infrequent and usually associated with pathological events. Here, we analyzed 40 pathogenic isolates of a haploid and asexual yeast, Candida glabrata, for their genome structure and stability. This organism has recently become the second most prevalent yeast pathogen in humans. Although the gene sequences were well conserved among different strains, their chromosome structures differed drastically. The most frequent events reshaping chromosomes were translocations of chromosomal arms. However, also larger segmental duplications were frequent and occasionally we observed novel chromosomes. Apparently, this yeast can generate a new chromosome by duplication of chromosome segments carrying a centromere and subsequently adding novel telomeric ends. We show that the observed genome plasticity is connected with antifungal drug resistance and it is likely an advantage in the human body, where environmental conditions fluctuate a lot. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Proceedings of the National Academy of Sciences
volume
106
issue
8
pages
2688 - 2693
publisher
National Acad Sciences
external identifiers
  • wos:000263652900041
  • scopus:62449137037
ISSN
1091-6490
DOI
10.1073/pnas.0809793106
language
English
LU publication?
yes
id
e058e8b0-a66f-4e63-8306-6a0a0bc6ca5a (old id 1302741)
date added to LUP
2009-03-27 13:38:17
date last changed
2017-12-10 03:46:00
@article{e058e8b0-a66f-4e63-8306-6a0a0bc6ca5a,
  abstract     = {In eukaryotes, the number and rough organization of chromosomes is well preserved within isolates of the same species. Novel chromosomes and loss of chromosomes are infrequent and usually associated with pathological events. Here, we analyzed 40 pathogenic isolates of a haploid and asexual yeast, Candida glabrata, for their genome structure and stability. This organism has recently become the second most prevalent yeast pathogen in humans. Although the gene sequences were well conserved among different strains, their chromosome structures differed drastically. The most frequent events reshaping chromosomes were translocations of chromosomal arms. However, also larger segmental duplications were frequent and occasionally we observed novel chromosomes. Apparently, this yeast can generate a new chromosome by duplication of chromosome segments carrying a centromere and subsequently adding novel telomeric ends. We show that the observed genome plasticity is connected with antifungal drug resistance and it is likely an advantage in the human body, where environmental conditions fluctuate a lot.},
  author       = {Poláková, Silvia and Blume, Christian and Zárate, Julián Alvarez and Mentel, Marek and Jørck-Ramberg, Dorte and Stenderup, Jørgen and Piskur, Jure},
  issn         = {1091-6490},
  language     = {eng},
  number       = {8},
  pages        = {2688--2693},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Formation of new chromosomes as a virulence mechanism in yeast Candida glabrata.},
  url          = {http://dx.doi.org/10.1073/pnas.0809793106},
  volume       = {106},
  year         = {2009},
}