Advanced

Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis.

Roybon, Laurent LU ; Deierborg, Tomas LU ; Brundin, Patrik LU and Li, Jia-Yi LU (2009) In European Journal of Neuroscience 29(2). p.232-243
Abstract
Postnatal neurogenesis takes place in two brain regions, the hippocampus and the subventricular zone (SVZ). The transcriptional cascade controlling hippocampal neurogenesis has been described in detail; however, the transcriptional control of olfactory bulb neurogenesis is still not well mapped. In this study, we provide insights into the molecular events controlling postnatal olfactory bulb neurogenesis. We first show the existence of diverse neural stem cell/progenitor populations along the SVZ-rostral migratory stream (RMS) axis, focusing on those expressing the basic helix-loop-helix (bHLH) transcription factor Mash1. We provide evidence that Mash1-derived progenies generate oligodendrocytic and neuronal precursors through the... (More)
Postnatal neurogenesis takes place in two brain regions, the hippocampus and the subventricular zone (SVZ). The transcriptional cascade controlling hippocampal neurogenesis has been described in detail; however, the transcriptional control of olfactory bulb neurogenesis is still not well mapped. In this study, we provide insights into the molecular events controlling postnatal olfactory bulb neurogenesis. We first show the existence of diverse neural stem cell/progenitor populations along the SVZ-rostral migratory stream (RMS) axis, focusing on those expressing the basic helix-loop-helix (bHLH) transcription factor Mash1. We provide evidence that Mash1-derived progenies generate oligodendrocytic and neuronal precursors through the transient expression of the bHLH transcription factors Olig2 and neurogenin2 (Ngn2), respectively. Furthermore, we reveal that Ngn2-positive progenies express the T-box transcription factors Tbr2 and Tbr1, which are usually present during cortical and hippocampal glutamatergic neuronal differentiation. We also highlight a cell population expressing another bHLH transcription factor, neuroD1 (ND1). The ND1-positive cells are located in the SVZ-RMS axis and also co-express Tbr2, Tbr1 and neuroD2. The observations that these cells incorporate bromodeoxyuridine and express both doublecortin and polysialylated form of neural cell adhesion molecule suggest that they are newborn neurons. Finally, using an in vitro assay, we demonstrate that Ngn2 and ND1 equally and exclusively direct differentiation of Mash1-expressing precursors into calbindin-expressing and calretinin-expressing neurons, which are both neuronal subtypes normally found in the olfactory bulb. Taken together, our data illustrate that Ngn2, neuroD and Tbr transcription factors are involved in postnatal neurogenesis in the olfactory bulb. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Neuroscience
volume
29
issue
2
pages
232 - 243
publisher
Wiley-Blackwell
external identifiers
  • wos:000262515600003
  • pmid:19200230
  • scopus:58449121331
ISSN
1460-9568
DOI
10.1111/j.1460-9568.2008.06595.x
language
English
LU publication?
yes
id
cd7f6278-687b-4a1d-9f0d-d122c28d5b7f (old id 1302789)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19200230?dopt=Abstract
date added to LUP
2009-03-06 11:01:13
date last changed
2017-10-01 04:54:57
@article{cd7f6278-687b-4a1d-9f0d-d122c28d5b7f,
  abstract     = {Postnatal neurogenesis takes place in two brain regions, the hippocampus and the subventricular zone (SVZ). The transcriptional cascade controlling hippocampal neurogenesis has been described in detail; however, the transcriptional control of olfactory bulb neurogenesis is still not well mapped. In this study, we provide insights into the molecular events controlling postnatal olfactory bulb neurogenesis. We first show the existence of diverse neural stem cell/progenitor populations along the SVZ-rostral migratory stream (RMS) axis, focusing on those expressing the basic helix-loop-helix (bHLH) transcription factor Mash1. We provide evidence that Mash1-derived progenies generate oligodendrocytic and neuronal precursors through the transient expression of the bHLH transcription factors Olig2 and neurogenin2 (Ngn2), respectively. Furthermore, we reveal that Ngn2-positive progenies express the T-box transcription factors Tbr2 and Tbr1, which are usually present during cortical and hippocampal glutamatergic neuronal differentiation. We also highlight a cell population expressing another bHLH transcription factor, neuroD1 (ND1). The ND1-positive cells are located in the SVZ-RMS axis and also co-express Tbr2, Tbr1 and neuroD2. The observations that these cells incorporate bromodeoxyuridine and express both doublecortin and polysialylated form of neural cell adhesion molecule suggest that they are newborn neurons. Finally, using an in vitro assay, we demonstrate that Ngn2 and ND1 equally and exclusively direct differentiation of Mash1-expressing precursors into calbindin-expressing and calretinin-expressing neurons, which are both neuronal subtypes normally found in the olfactory bulb. Taken together, our data illustrate that Ngn2, neuroD and Tbr transcription factors are involved in postnatal neurogenesis in the olfactory bulb.},
  author       = {Roybon, Laurent and Deierborg, Tomas and Brundin, Patrik and Li, Jia-Yi},
  issn         = {1460-9568},
  language     = {eng},
  number       = {2},
  pages        = {232--243},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis.},
  url          = {http://dx.doi.org/10.1111/j.1460-9568.2008.06595.x},
  volume       = {29},
  year         = {2009},
}