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Identification of triacylglycerols in the enzymatic transesterification of rapeseed and butter oil

Svensson, Julia LU and Adlercreutz, Patrick LU orcid (2008) In European Journal of Lipid Science and Technology 110(11). p.1007-1013
Abstract
A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme (R) TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as alpha-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in... (More)
A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme (R) TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as alpha-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in the fatty acid composition in the sn-2 position were detected after 6 h. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Reversed-phase HPLC, Transesterification, HPLC-electrospray tandem mass spectrometry, Triacylglycerols, Lipozyme TL IM
in
European Journal of Lipid Science and Technology
volume
110
issue
11
pages
1007 - 1013
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000261456300005
  • scopus:56749182597
ISSN
1438-7697
DOI
10.1002/ejlt.200800074
language
English
LU publication?
yes
id
c3be9eb4-dce8-4805-a384-6110c051fda9 (old id 1305671)
date added to LUP
2016-04-01 12:19:11
date last changed
2022-01-27 01:56:16
@article{c3be9eb4-dce8-4805-a384-6110c051fda9,
  abstract     = {{A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme (R) TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as alpha-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in the fatty acid composition in the sn-2 position were detected after 6 h.}},
  author       = {{Svensson, Julia and Adlercreutz, Patrick}},
  issn         = {{1438-7697}},
  keywords     = {{Reversed-phase HPLC; Transesterification; HPLC-electrospray tandem mass spectrometry; Triacylglycerols; Lipozyme TL IM}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1007--1013}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{European Journal of Lipid Science and Technology}},
  title        = {{Identification of triacylglycerols in the enzymatic transesterification of rapeseed and butter oil}},
  url          = {{http://dx.doi.org/10.1002/ejlt.200800074}},
  doi          = {{10.1002/ejlt.200800074}},
  volume       = {{110}},
  year         = {{2008}},
}