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Identification of triacylglycerols in the enzymatic transesterification of rapeseed and butter oil

Svensson, Julia LU and Adlercreutz, Patrick LU (2008) In European Journal of Lipid Science and Technology 110(11). p.1007-1013
Abstract
A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme (R) TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as alpha-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in... (More)
A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme (R) TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as alpha-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in the fatty acid composition in the sn-2 position were detected after 6 h. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Reversed-phase HPLC, Transesterification, HPLC-electrospray tandem mass spectrometry, Triacylglycerols, Lipozyme TL IM
in
European Journal of Lipid Science and Technology
volume
110
issue
11
pages
1007 - 1013
publisher
John Wiley & Sons
external identifiers
  • wos:000261456300005
  • scopus:56749182597
ISSN
1438-7697
DOI
10.1002/ejlt.200800074
language
English
LU publication?
yes
id
c3be9eb4-dce8-4805-a384-6110c051fda9 (old id 1305671)
date added to LUP
2009-03-23 12:11:57
date last changed
2017-09-03 03:51:33
@article{c3be9eb4-dce8-4805-a384-6110c051fda9,
  abstract     = {A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme (R) TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as alpha-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in the fatty acid composition in the sn-2 position were detected after 6 h.},
  author       = {Svensson, Julia and Adlercreutz, Patrick},
  issn         = {1438-7697},
  keyword      = {Reversed-phase HPLC,Transesterification,HPLC-electrospray tandem mass spectrometry,Triacylglycerols,Lipozyme TL IM},
  language     = {eng},
  number       = {11},
  pages        = {1007--1013},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Lipid Science and Technology},
  title        = {Identification of triacylglycerols in the enzymatic transesterification of rapeseed and butter oil},
  url          = {http://dx.doi.org/10.1002/ejlt.200800074},
  volume       = {110},
  year         = {2008},
}