Alterations of Notch/Jagged mRNA and protein expression after partial hepatectomy in rats
(2008) In Scandinavian Journal of Gastroenterology 43(12). p.1522-1528- Abstract
- Objective. To investigate alterations of tumor necrosis factor (TNF)-, interleukin (IL)-6, the Notch/Jagged signaling pathway, and hepatocyte proliferation, and their correlation in rats subjected to partial hepatectomy. Material and methods. Blood and liver tissues were sampled immediately (0), 5, 15, 30, 60, 180, 360, and 720 min, and 1, 2, 4, and 7 days after partial (70%) hepatectomy to measure plasma levels of TNF- and IL-6, the expression of proliferating cell nuclear antigen (PCNA), Notch-1 and Jagged-1 protein, and mRNA in liver tissue. Results. Systemic levels of IL-6 significantly correlated with the expression of PCNA in hepatocytes (p0.05) after partial (70%) hepatectomy. The increased expression of Notch-1 protein was located... (More)
- Objective. To investigate alterations of tumor necrosis factor (TNF)-, interleukin (IL)-6, the Notch/Jagged signaling pathway, and hepatocyte proliferation, and their correlation in rats subjected to partial hepatectomy. Material and methods. Blood and liver tissues were sampled immediately (0), 5, 15, 30, 60, 180, 360, and 720 min, and 1, 2, 4, and 7 days after partial (70%) hepatectomy to measure plasma levels of TNF- and IL-6, the expression of proliferating cell nuclear antigen (PCNA), Notch-1 and Jagged-1 protein, and mRNA in liver tissue. Results. Systemic levels of IL-6 significantly correlated with the expression of PCNA in hepatocytes (p0.05) after partial (70%) hepatectomy. The increased expression of Notch-1 protein was located in the sinusoidal endothelium, while Jagged-1 protein was predominantly located in bile duct cells. Expression of both proteins was increased around the portal vein. mRNA expression of Notch-1 decreased between 6 h and 2 days after partial hepatectomy, while Jagged-1 increased between 3 and 6 h, decreased between 12 h and 2 days, and normalized on day 4 after hepatectomy. Conclusions. Partial hepatectomy-induced hepatocyte proliferation was accompanied by an increase in systemic levels of IL-6, a pro-inflammatory response that plays a part in the proliferative process. The Notch/Jagged signaling pathways are activated and probably represent important contributors to the regenerative process following hepatectomy including both hepatocyte proliferation and bile duct formation and growth. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1305838
- author
- Xu, Hong Yu ; Li, Bao Jie ; Wang, Rui Feng and Andersson, Roland LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- hepatectomy, Jagged proteins, proliferation, Notch, Cytokines
- in
- Scandinavian Journal of Gastroenterology
- volume
- 43
- issue
- 12
- pages
- 1522 - 1528
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000261174700017
- scopus:56749107385
- pmid:18781453
- ISSN
- 1502-7708
- DOI
- 10.1080/00365520802321188
- language
- English
- LU publication?
- yes
- id
- 8cd12969-e13e-4ce2-8223-7fcb113b6aca (old id 1305838)
- date added to LUP
- 2016-04-01 13:07:51
- date last changed
- 2022-01-27 17:28:46
@article{8cd12969-e13e-4ce2-8223-7fcb113b6aca,
abstract = {{Objective. To investigate alterations of tumor necrosis factor (TNF)-, interleukin (IL)-6, the Notch/Jagged signaling pathway, and hepatocyte proliferation, and their correlation in rats subjected to partial hepatectomy. Material and methods. Blood and liver tissues were sampled immediately (0), 5, 15, 30, 60, 180, 360, and 720 min, and 1, 2, 4, and 7 days after partial (70%) hepatectomy to measure plasma levels of TNF- and IL-6, the expression of proliferating cell nuclear antigen (PCNA), Notch-1 and Jagged-1 protein, and mRNA in liver tissue. Results. Systemic levels of IL-6 significantly correlated with the expression of PCNA in hepatocytes (p0.05) after partial (70%) hepatectomy. The increased expression of Notch-1 protein was located in the sinusoidal endothelium, while Jagged-1 protein was predominantly located in bile duct cells. Expression of both proteins was increased around the portal vein. mRNA expression of Notch-1 decreased between 6 h and 2 days after partial hepatectomy, while Jagged-1 increased between 3 and 6 h, decreased between 12 h and 2 days, and normalized on day 4 after hepatectomy. Conclusions. Partial hepatectomy-induced hepatocyte proliferation was accompanied by an increase in systemic levels of IL-6, a pro-inflammatory response that plays a part in the proliferative process. The Notch/Jagged signaling pathways are activated and probably represent important contributors to the regenerative process following hepatectomy including both hepatocyte proliferation and bile duct formation and growth.}},
author = {{Xu, Hong Yu and Li, Bao Jie and Wang, Rui Feng and Andersson, Roland}},
issn = {{1502-7708}},
keywords = {{hepatectomy; Jagged proteins; proliferation; Notch; Cytokines}},
language = {{eng}},
number = {{12}},
pages = {{1522--1528}},
publisher = {{Taylor & Francis}},
series = {{Scandinavian Journal of Gastroenterology}},
title = {{Alterations of Notch/Jagged mRNA and protein expression after partial hepatectomy in rats}},
url = {{http://dx.doi.org/10.1080/00365520802321188}},
doi = {{10.1080/00365520802321188}},
volume = {{43}},
year = {{2008}},
}