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Alterations of Notch/Jagged mRNA and protein expression after partial hepatectomy in rats

Xu, Hong Yu; Li, Bao Jie; Wang, Rui Feng and Andersson, Roland LU (2008) In Scandinavian Journal of Gastroenterology 43(12). p.1522-1528
Abstract
Objective. To investigate alterations of tumor necrosis factor (TNF)-, interleukin (IL)-6, the Notch/Jagged signaling pathway, and hepatocyte proliferation, and their correlation in rats subjected to partial hepatectomy. Material and methods. Blood and liver tissues were sampled immediately (0), 5, 15, 30, 60, 180, 360, and 720 min, and 1, 2, 4, and 7 days after partial (70%) hepatectomy to measure plasma levels of TNF- and IL-6, the expression of proliferating cell nuclear antigen (PCNA), Notch-1 and Jagged-1 protein, and mRNA in liver tissue. Results. Systemic levels of IL-6 significantly correlated with the expression of PCNA in hepatocytes (p0.05) after partial (70%) hepatectomy. The increased expression of Notch-1 protein was located... (More)
Objective. To investigate alterations of tumor necrosis factor (TNF)-, interleukin (IL)-6, the Notch/Jagged signaling pathway, and hepatocyte proliferation, and their correlation in rats subjected to partial hepatectomy. Material and methods. Blood and liver tissues were sampled immediately (0), 5, 15, 30, 60, 180, 360, and 720 min, and 1, 2, 4, and 7 days after partial (70%) hepatectomy to measure plasma levels of TNF- and IL-6, the expression of proliferating cell nuclear antigen (PCNA), Notch-1 and Jagged-1 protein, and mRNA in liver tissue. Results. Systemic levels of IL-6 significantly correlated with the expression of PCNA in hepatocytes (p0.05) after partial (70%) hepatectomy. The increased expression of Notch-1 protein was located in the sinusoidal endothelium, while Jagged-1 protein was predominantly located in bile duct cells. Expression of both proteins was increased around the portal vein. mRNA expression of Notch-1 decreased between 6 h and 2 days after partial hepatectomy, while Jagged-1 increased between 3 and 6 h, decreased between 12 h and 2 days, and normalized on day 4 after hepatectomy. Conclusions. Partial hepatectomy-induced hepatocyte proliferation was accompanied by an increase in systemic levels of IL-6, a pro-inflammatory response that plays a part in the proliferative process. The Notch/Jagged signaling pathways are activated and probably represent important contributors to the regenerative process following hepatectomy including both hepatocyte proliferation and bile duct formation and growth. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hepatectomy, Jagged proteins, proliferation, Notch, Cytokines
in
Scandinavian Journal of Gastroenterology
volume
43
issue
12
pages
1522 - 1528
publisher
Taylor & Francis
external identifiers
  • wos:000261174700017
  • scopus:56749107385
ISSN
1502-7708
DOI
10.1080/00365520802321188
language
English
LU publication?
yes
id
8cd12969-e13e-4ce2-8223-7fcb113b6aca (old id 1305838)
date added to LUP
2009-03-23 09:42:08
date last changed
2017-01-01 05:28:59
@article{8cd12969-e13e-4ce2-8223-7fcb113b6aca,
  abstract     = {Objective. To investigate alterations of tumor necrosis factor (TNF)-, interleukin (IL)-6, the Notch/Jagged signaling pathway, and hepatocyte proliferation, and their correlation in rats subjected to partial hepatectomy. Material and methods. Blood and liver tissues were sampled immediately (0), 5, 15, 30, 60, 180, 360, and 720 min, and 1, 2, 4, and 7 days after partial (70%) hepatectomy to measure plasma levels of TNF- and IL-6, the expression of proliferating cell nuclear antigen (PCNA), Notch-1 and Jagged-1 protein, and mRNA in liver tissue. Results. Systemic levels of IL-6 significantly correlated with the expression of PCNA in hepatocytes (p0.05) after partial (70%) hepatectomy. The increased expression of Notch-1 protein was located in the sinusoidal endothelium, while Jagged-1 protein was predominantly located in bile duct cells. Expression of both proteins was increased around the portal vein. mRNA expression of Notch-1 decreased between 6 h and 2 days after partial hepatectomy, while Jagged-1 increased between 3 and 6 h, decreased between 12 h and 2 days, and normalized on day 4 after hepatectomy. Conclusions. Partial hepatectomy-induced hepatocyte proliferation was accompanied by an increase in systemic levels of IL-6, a pro-inflammatory response that plays a part in the proliferative process. The Notch/Jagged signaling pathways are activated and probably represent important contributors to the regenerative process following hepatectomy including both hepatocyte proliferation and bile duct formation and growth.},
  author       = {Xu, Hong Yu and Li, Bao Jie and Wang, Rui Feng and Andersson, Roland},
  issn         = {1502-7708},
  keyword      = {hepatectomy,Jagged proteins,proliferation,Notch,Cytokines},
  language     = {eng},
  number       = {12},
  pages        = {1522--1528},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Alterations of Notch/Jagged mRNA and protein expression after partial hepatectomy in rats},
  url          = {http://dx.doi.org/10.1080/00365520802321188},
  volume       = {43},
  year         = {2008},
}