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The effects of the loss of TIP1;1 and TIP1;2 aquaporins in Arabidopsis thaliana

Schussler, Manuela Desiree; Alexandersson, Erik LU ; Bienert, Gerd Patrick; Kichey, Thomas; Laursen, Kristian Holst; Johanson, Urban LU ; Kjellbom, Per LU ; Schjoerring, Jan Kofod and Jahn, Thomas Paul (2008) In Plant Journal 56(5). p.756-767
Abstract
Loss of aquaporin TIP1;1 in Arabidopsis has been suggested to result in early senescence and plant death. This was based on the fact that a partial reduction of TIP1;1 by RNA interference (RNAi) led to gradual phenotypes, ranging from indistinguishable from wild type to lethality, depending on the degree of downregulation of the target messenger, and displaying pleiotropic effects in primary metabolism and cell signalling. A hypothesis was put forward to suggest that TIP1;1, apart from its transport function, may play an essential role in vesicle routing. Here we identify an Arabidopsis transposon insertion line tip1;1-1 that is completely devoid of TIP1;1 protein, as demonstrated by western blotting and immunolocalization using an... (More)
Loss of aquaporin TIP1;1 in Arabidopsis has been suggested to result in early senescence and plant death. This was based on the fact that a partial reduction of TIP1;1 by RNA interference (RNAi) led to gradual phenotypes, ranging from indistinguishable from wild type to lethality, depending on the degree of downregulation of the target messenger, and displaying pleiotropic effects in primary metabolism and cell signalling. A hypothesis was put forward to suggest that TIP1;1, apart from its transport function, may play an essential role in vesicle routing. Here we identify an Arabidopsis transposon insertion line tip1;1-1 that is completely devoid of TIP1;1 protein, as demonstrated by western blotting and immunolocalization using an isoform-specific antibody. Strikingly, the complete absence of the protein did not result in any significant effect on metabolism or elemental composition of the plants. Microarray analysis did not indicate increased expression of other aquaporins to compensate for the lack of TIP1;1 in tip1;1-1. We further developed a double mutant of TIPs in Arabidopsis, lacking both TIP1;1 and its closest paralog TIP1;2. Arabidopsis mutants lacking both TIP1;1 and TIP1;2 showed a minor increase in anthocyanin content, and a reduction in catalase activity, but showed no changes in water status. In contrast to earlier reports, plants lacking TIP1;1 and TIP1;2 aquaporins are alive and thriving. We suggest that RNAi directed towards TIP1;1 may have resulted in off-target gene silencing, a notion that is potentially interesting for various studies analysing gene function by RNAi. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
insertion line, RNAi, aquaporin, TIP1, off-targeting
in
Plant Journal
volume
56
issue
5
pages
756 - 767
publisher
Wiley-Blackwell
external identifiers
  • wos:000261275400006
  • scopus:57449084176
ISSN
1365-313X
DOI
10.1111/j.1365-313X.2008.03632.x
language
English
LU publication?
yes
id
e189fdb5-1d25-40c3-8bc3-e62601f19ee7 (old id 1305856)
date added to LUP
2009-03-20 11:09:56
date last changed
2017-10-01 04:23:54
@article{e189fdb5-1d25-40c3-8bc3-e62601f19ee7,
  abstract     = {Loss of aquaporin TIP1;1 in Arabidopsis has been suggested to result in early senescence and plant death. This was based on the fact that a partial reduction of TIP1;1 by RNA interference (RNAi) led to gradual phenotypes, ranging from indistinguishable from wild type to lethality, depending on the degree of downregulation of the target messenger, and displaying pleiotropic effects in primary metabolism and cell signalling. A hypothesis was put forward to suggest that TIP1;1, apart from its transport function, may play an essential role in vesicle routing. Here we identify an Arabidopsis transposon insertion line tip1;1-1 that is completely devoid of TIP1;1 protein, as demonstrated by western blotting and immunolocalization using an isoform-specific antibody. Strikingly, the complete absence of the protein did not result in any significant effect on metabolism or elemental composition of the plants. Microarray analysis did not indicate increased expression of other aquaporins to compensate for the lack of TIP1;1 in tip1;1-1. We further developed a double mutant of TIPs in Arabidopsis, lacking both TIP1;1 and its closest paralog TIP1;2. Arabidopsis mutants lacking both TIP1;1 and TIP1;2 showed a minor increase in anthocyanin content, and a reduction in catalase activity, but showed no changes in water status. In contrast to earlier reports, plants lacking TIP1;1 and TIP1;2 aquaporins are alive and thriving. We suggest that RNAi directed towards TIP1;1 may have resulted in off-target gene silencing, a notion that is potentially interesting for various studies analysing gene function by RNAi.},
  author       = {Schussler, Manuela Desiree and Alexandersson, Erik and Bienert, Gerd Patrick and Kichey, Thomas and Laursen, Kristian Holst and Johanson, Urban and Kjellbom, Per and Schjoerring, Jan Kofod and Jahn, Thomas Paul},
  issn         = {1365-313X},
  keyword      = {insertion line,RNAi,aquaporin,TIP1,off-targeting},
  language     = {eng},
  number       = {5},
  pages        = {756--767},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {The effects of the loss of TIP1;1 and TIP1;2 aquaporins in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2008.03632.x},
  volume       = {56},
  year         = {2008},
}