Advanced

Quality aspects of the tissue microarray technique in a population-based cohort with ductal carcinoma in situ of the breast

Warnberg, F.; Amini, R-M; Goldman, M and Jirström, Karin LU (2008) In Histopathology 53(6). p.642-649
Abstract
Tissue microarray (TMA) is an efficient technique for analysis of molecular markers. Prospectively collected samples have been reported to give excellent concordance between TMA data and corresponding whole-sections. The aim was to evaluate the usefulness of TMA in a population-based cohort of 213 women with ductal carcinoma in situ of the breast (DCIS). We studied immunohistochemical HER2, oestrogen (ER) and progesterone (PR) receptor status. The prognostic impact was similar for all markers comparing whole sections and TMAs. The proportion of positive tumours was similar regarding HER2 and ER, whereas PR tumours were more frequently positive in the TMAs (P = 0.007). The concordance was 80% (kappa value 0.63) between original sections and... (More)
Tissue microarray (TMA) is an efficient technique for analysis of molecular markers. Prospectively collected samples have been reported to give excellent concordance between TMA data and corresponding whole-sections. The aim was to evaluate the usefulness of TMA in a population-based cohort of 213 women with ductal carcinoma in situ of the breast (DCIS). We studied immunohistochemical HER2, oestrogen (ER) and progesterone (PR) receptor status. The prognostic impact was similar for all markers comparing whole sections and TMAs. The proportion of positive tumours was similar regarding HER2 and ER, whereas PR tumours were more frequently positive in the TMAs (P = 0.007). The concordance was 80% (kappa value 0.63) between original sections and TMAs. The proportion of successfully analysed tumours was 70%. Smaller tumours had a lower ratio (P < 0.0001) and a larger proportion of mismatched results (P = 0.05). Retrospective analyses of tumours from cohorts with long-term follow-up are indispensable. We have shown that the TMA technique is a useful tool for high-throughput analysis of DCIS. However, our study has pinpointed some technical hazards within a population-based cohort, including many small lesions and the poor condition of some donor blocks. Mismatched results may be due to tumour heterogeneity. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tissue microarray, quality, prognosis, in situ, breast, cancer
in
Histopathology
volume
53
issue
6
pages
642 - 649
publisher
Wiley-Blackwell
external identifiers
  • wos:000261251300003
  • scopus:57049089023
ISSN
0309-0167
DOI
10.1111/j.1365-2559.2008.03156.x
language
English
LU publication?
yes
id
2798a4bb-c956-406a-b187-b777137cd210 (old id 1307579)
date added to LUP
2009-03-20 08:35:12
date last changed
2017-11-12 03:22:43
@article{2798a4bb-c956-406a-b187-b777137cd210,
  abstract     = {Tissue microarray (TMA) is an efficient technique for analysis of molecular markers. Prospectively collected samples have been reported to give excellent concordance between TMA data and corresponding whole-sections. The aim was to evaluate the usefulness of TMA in a population-based cohort of 213 women with ductal carcinoma in situ of the breast (DCIS). We studied immunohistochemical HER2, oestrogen (ER) and progesterone (PR) receptor status. The prognostic impact was similar for all markers comparing whole sections and TMAs. The proportion of positive tumours was similar regarding HER2 and ER, whereas PR tumours were more frequently positive in the TMAs (P = 0.007). The concordance was 80% (kappa value 0.63) between original sections and TMAs. The proportion of successfully analysed tumours was 70%. Smaller tumours had a lower ratio (P &lt; 0.0001) and a larger proportion of mismatched results (P = 0.05). Retrospective analyses of tumours from cohorts with long-term follow-up are indispensable. We have shown that the TMA technique is a useful tool for high-throughput analysis of DCIS. However, our study has pinpointed some technical hazards within a population-based cohort, including many small lesions and the poor condition of some donor blocks. Mismatched results may be due to tumour heterogeneity.},
  author       = {Warnberg, F. and Amini, R-M and Goldman, M and Jirström, Karin},
  issn         = {0309-0167},
  keyword      = {tissue microarray,quality,prognosis,in situ,breast,cancer},
  language     = {eng},
  number       = {6},
  pages        = {642--649},
  publisher    = {Wiley-Blackwell},
  series       = {Histopathology},
  title        = {Quality aspects of the tissue microarray technique in a population-based cohort with ductal carcinoma in situ of the breast},
  url          = {http://dx.doi.org/10.1111/j.1365-2559.2008.03156.x},
  volume       = {53},
  year         = {2008},
}