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Effect of tertiary sewage effluent additions on Prymnesium parvum cell toxicity and stable isotope ratios

Lindehoff, Elin; Graneli, Edna and Granéli, Wilhelm LU (2009) In Harmful Algae 8(2). p.247-253
Abstract
We investigated the ability of the ichthyotoxic haptophyte Prymnesium parvum to use sewage-originated nutrients applying stable carbon (C) and nitrogen (N) isotope techniques. P. parvum was cultured under N and phosphorus (P) sufficient and deficient conditions in either sewage effluent-based medium or in a nitrate- and phosphate-based control. Cell densities and toxicities were monitored and stable carbon N isotopes signatures (delta C-13 and delta N-15) of P. parvum and the sewage effluent analysed. Nitrogen and P sufficient cultures achieved the highest biomass followed by P and N deficient cultures, regardless of sewage effluent additions. The P deficient cultures with sewage effluent had higher toxicity, estimated as haemolytic... (More)
We investigated the ability of the ichthyotoxic haptophyte Prymnesium parvum to use sewage-originated nutrients applying stable carbon (C) and nitrogen (N) isotope techniques. P. parvum was cultured under N and phosphorus (P) sufficient and deficient conditions in either sewage effluent-based medium or in a nitrate- and phosphate-based control. Cell densities and toxicities were monitored and stable carbon N isotopes signatures (delta C-13 and delta N-15) of P. parvum and the sewage effluent analysed. Nitrogen and P sufficient cultures achieved the highest biomass followed by P and N deficient cultures, regardless of sewage effluent additions. The P deficient cultures with sewage effluent had higher toxicity, estimated as haemolytic activity (9.4 +/- 0 x 10(-5) mg Saponin equiv. cell(-1)) compared to the P deficient control and to all N deficient and NP sufficient cultures. Nutrient deficient conditions had no effect on the cell delta N-15, but a decreasing effect on delta C-13 in the inorganic N deficient treatment. Growth in sewage-based media was followed by a substantial increase in the cell delta N-15 (10.4-16.1.60) compared to the control treatments (2.4-4.9%o), showing that P. parvum is capable of direct use of sewage-originated N, inorganic as well as organic. Uptake of terrestrial derived C in the sewage treatments was confirmed by a decrease in cell delta C-13, implying that P. parvum is able to utilize organic nutrients in sewage effluent. (C) 2008 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
isotope, Sewage effluent, Toxicity, Stable carbon and nitrogen, Nutrient deficiency, Prymnesium parvum
in
Harmful Algae
volume
8
issue
2
pages
247 - 253
publisher
Elsevier
external identifiers
  • wos:000262803800008
  • scopus:57649202269
ISSN
1878-1470
DOI
10.1016/j.hal.2008.06.004
language
English
LU publication?
yes
id
e910beaf-6201-42cb-a0c6-25f17a1a692a (old id 1311501)
date added to LUP
2009-03-17 10:15:33
date last changed
2017-01-01 04:37:25
@article{e910beaf-6201-42cb-a0c6-25f17a1a692a,
  abstract     = {We investigated the ability of the ichthyotoxic haptophyte Prymnesium parvum to use sewage-originated nutrients applying stable carbon (C) and nitrogen (N) isotope techniques. P. parvum was cultured under N and phosphorus (P) sufficient and deficient conditions in either sewage effluent-based medium or in a nitrate- and phosphate-based control. Cell densities and toxicities were monitored and stable carbon N isotopes signatures (delta C-13 and delta N-15) of P. parvum and the sewage effluent analysed. Nitrogen and P sufficient cultures achieved the highest biomass followed by P and N deficient cultures, regardless of sewage effluent additions. The P deficient cultures with sewage effluent had higher toxicity, estimated as haemolytic activity (9.4 +/- 0 x 10(-5) mg Saponin equiv. cell(-1)) compared to the P deficient control and to all N deficient and NP sufficient cultures. Nutrient deficient conditions had no effect on the cell delta N-15, but a decreasing effect on delta C-13 in the inorganic N deficient treatment. Growth in sewage-based media was followed by a substantial increase in the cell delta N-15 (10.4-16.1.60) compared to the control treatments (2.4-4.9%o), showing that P. parvum is capable of direct use of sewage-originated N, inorganic as well as organic. Uptake of terrestrial derived C in the sewage treatments was confirmed by a decrease in cell delta C-13, implying that P. parvum is able to utilize organic nutrients in sewage effluent. (C) 2008 Elsevier B.V. All rights reserved.},
  author       = {Lindehoff, Elin and Graneli, Edna and Granéli, Wilhelm},
  issn         = {1878-1470},
  keyword      = {isotope,Sewage effluent,Toxicity,Stable carbon and nitrogen,Nutrient deficiency,Prymnesium parvum},
  language     = {eng},
  number       = {2},
  pages        = {247--253},
  publisher    = {Elsevier},
  series       = {Harmful Algae},
  title        = {Effect of tertiary sewage effluent additions on Prymnesium parvum cell toxicity and stable isotope ratios},
  url          = {http://dx.doi.org/10.1016/j.hal.2008.06.004},
  volume       = {8},
  year         = {2009},
}