Effect of tertiary sewage effluent additions on Prymnesium parvum cell toxicity and stable isotope ratios
(2009) In Harmful Algae 8(2). p.247-253- Abstract
- We investigated the ability of the ichthyotoxic haptophyte Prymnesium parvum to use sewage-originated nutrients applying stable carbon (C) and nitrogen (N) isotope techniques. P. parvum was cultured under N and phosphorus (P) sufficient and deficient conditions in either sewage effluent-based medium or in a nitrate- and phosphate-based control. Cell densities and toxicities were monitored and stable carbon N isotopes signatures (delta C-13 and delta N-15) of P. parvum and the sewage effluent analysed. Nitrogen and P sufficient cultures achieved the highest biomass followed by P and N deficient cultures, regardless of sewage effluent additions. The P deficient cultures with sewage effluent had higher toxicity, estimated as haemolytic... (More)
- We investigated the ability of the ichthyotoxic haptophyte Prymnesium parvum to use sewage-originated nutrients applying stable carbon (C) and nitrogen (N) isotope techniques. P. parvum was cultured under N and phosphorus (P) sufficient and deficient conditions in either sewage effluent-based medium or in a nitrate- and phosphate-based control. Cell densities and toxicities were monitored and stable carbon N isotopes signatures (delta C-13 and delta N-15) of P. parvum and the sewage effluent analysed. Nitrogen and P sufficient cultures achieved the highest biomass followed by P and N deficient cultures, regardless of sewage effluent additions. The P deficient cultures with sewage effluent had higher toxicity, estimated as haemolytic activity (9.4 +/- 0 x 10(-5) mg Saponin equiv. cell(-1)) compared to the P deficient control and to all N deficient and NP sufficient cultures. Nutrient deficient conditions had no effect on the cell delta N-15, but a decreasing effect on delta C-13 in the inorganic N deficient treatment. Growth in sewage-based media was followed by a substantial increase in the cell delta N-15 (10.4-16.1.60) compared to the control treatments (2.4-4.9%o), showing that P. parvum is capable of direct use of sewage-originated N, inorganic as well as organic. Uptake of terrestrial derived C in the sewage treatments was confirmed by a decrease in cell delta C-13, implying that P. parvum is able to utilize organic nutrients in sewage effluent. (C) 2008 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1311501
- author
- Lindehoff, Elin ; Graneli, Edna and Granéli, Wilhelm LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- isotope, Sewage effluent, Toxicity, Stable carbon and nitrogen, Nutrient deficiency, Prymnesium parvum
- in
- Harmful Algae
- volume
- 8
- issue
- 2
- pages
- 247 - 253
- publisher
- Elsevier
- external identifiers
-
- wos:000262803800008
- scopus:57649202269
- ISSN
- 1878-1470
- DOI
- 10.1016/j.hal.2008.06.004
- language
- English
- LU publication?
- yes
- id
- e910beaf-6201-42cb-a0c6-25f17a1a692a (old id 1311501)
- date added to LUP
- 2016-04-01 11:52:26
- date last changed
- 2022-01-26 19:31:37
@article{e910beaf-6201-42cb-a0c6-25f17a1a692a,
abstract = {{We investigated the ability of the ichthyotoxic haptophyte Prymnesium parvum to use sewage-originated nutrients applying stable carbon (C) and nitrogen (N) isotope techniques. P. parvum was cultured under N and phosphorus (P) sufficient and deficient conditions in either sewage effluent-based medium or in a nitrate- and phosphate-based control. Cell densities and toxicities were monitored and stable carbon N isotopes signatures (delta C-13 and delta N-15) of P. parvum and the sewage effluent analysed. Nitrogen and P sufficient cultures achieved the highest biomass followed by P and N deficient cultures, regardless of sewage effluent additions. The P deficient cultures with sewage effluent had higher toxicity, estimated as haemolytic activity (9.4 +/- 0 x 10(-5) mg Saponin equiv. cell(-1)) compared to the P deficient control and to all N deficient and NP sufficient cultures. Nutrient deficient conditions had no effect on the cell delta N-15, but a decreasing effect on delta C-13 in the inorganic N deficient treatment. Growth in sewage-based media was followed by a substantial increase in the cell delta N-15 (10.4-16.1.60) compared to the control treatments (2.4-4.9%o), showing that P. parvum is capable of direct use of sewage-originated N, inorganic as well as organic. Uptake of terrestrial derived C in the sewage treatments was confirmed by a decrease in cell delta C-13, implying that P. parvum is able to utilize organic nutrients in sewage effluent. (C) 2008 Elsevier B.V. All rights reserved.}},
author = {{Lindehoff, Elin and Graneli, Edna and Granéli, Wilhelm}},
issn = {{1878-1470}},
keywords = {{isotope; Sewage effluent; Toxicity; Stable carbon and nitrogen; Nutrient deficiency; Prymnesium parvum}},
language = {{eng}},
number = {{2}},
pages = {{247--253}},
publisher = {{Elsevier}},
series = {{Harmful Algae}},
title = {{Effect of tertiary sewage effluent additions on Prymnesium parvum cell toxicity and stable isotope ratios}},
url = {{http://dx.doi.org/10.1016/j.hal.2008.06.004}},
doi = {{10.1016/j.hal.2008.06.004}},
volume = {{8}},
year = {{2009}},
}