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Novel Vinylpyridine Based Cationic MIP Monoliths for Enantiomer Separation in CEC

Spégel, Peter LU ; Schweitz, Leif; Andersson, Lars I. and Nilsson, Staffan LU (2009) In Chromatographia 69(3-4). p.277-285
Abstract
Cationic vinylpyridine based molecularly imprinted polymer monoliths were, for the first time, applied to enantiomer separation using capillary electrochromatography. In order to map the synthesis conditions under which superporous monoliths are obtained, capillary columns were prepared by an in situ polymerization technique using varying monomer and porogen compositions. Both electrolyte pH and percentage of the organic modifier acetonitrile were found to affect the electrochromatographic behaviour on such columns. An interesting observation is that the electroosmotic flow changed direction from cathodic at high pH to anodic at low pH of the electrolyte, opening up the opportunity to manipulate the flow. This is attributed to the... (More)
Cationic vinylpyridine based molecularly imprinted polymer monoliths were, for the first time, applied to enantiomer separation using capillary electrochromatography. In order to map the synthesis conditions under which superporous monoliths are obtained, capillary columns were prepared by an in situ polymerization technique using varying monomer and porogen compositions. Both electrolyte pH and percentage of the organic modifier acetonitrile were found to affect the electrochromatographic behaviour on such columns. An interesting observation is that the electroosmotic flow changed direction from cathodic at high pH to anodic at low pH of the electrolyte, opening up the opportunity to manipulate the flow. This is attributed to the vinylpyridine based MIP becomes protonated and positively charged at low pH, whereas it is uncharged at high pH where instead negatively charged silanol groups drive the electroosmotic flow. Improved enantiomer resolution, as well as increased retention, was observed when the concentration of acetonitrile in the electrolyte decreased, indicating a significant element of hydrophobic effects in the molecular recognition of the imprinted enantiomer. Enantiomer separation of the non-steroidal anti-inflammatory drug ibuprofen was studied and efficiencies as high as 30,000 plates per meter with an asymmetry factor below 4 were obtained for the last eluting imprinted enantiomer. These values are better than that obtained generally using MIP based chromatography. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary electrochromatography, Molecularly imprinted polymer, Monolith, Vinylpyridine, Ibuprofen
in
Chromatographia
volume
69
issue
3-4
pages
277 - 285
publisher
Vieweg Verlag
external identifiers
  • wos:000262967600012
  • scopus:59449105481
ISSN
0009-5893
DOI
10.1365/s10337-008-0911-0
language
English
LU publication?
yes
id
2ce517f7-432b-4fbd-9e99-b173240891ab (old id 1311571)
date added to LUP
2009-03-17 09:33:51
date last changed
2017-01-01 05:37:18
@article{2ce517f7-432b-4fbd-9e99-b173240891ab,
  abstract     = {Cationic vinylpyridine based molecularly imprinted polymer monoliths were, for the first time, applied to enantiomer separation using capillary electrochromatography. In order to map the synthesis conditions under which superporous monoliths are obtained, capillary columns were prepared by an in situ polymerization technique using varying monomer and porogen compositions. Both electrolyte pH and percentage of the organic modifier acetonitrile were found to affect the electrochromatographic behaviour on such columns. An interesting observation is that the electroosmotic flow changed direction from cathodic at high pH to anodic at low pH of the electrolyte, opening up the opportunity to manipulate the flow. This is attributed to the vinylpyridine based MIP becomes protonated and positively charged at low pH, whereas it is uncharged at high pH where instead negatively charged silanol groups drive the electroosmotic flow. Improved enantiomer resolution, as well as increased retention, was observed when the concentration of acetonitrile in the electrolyte decreased, indicating a significant element of hydrophobic effects in the molecular recognition of the imprinted enantiomer. Enantiomer separation of the non-steroidal anti-inflammatory drug ibuprofen was studied and efficiencies as high as 30,000 plates per meter with an asymmetry factor below 4 were obtained for the last eluting imprinted enantiomer. These values are better than that obtained generally using MIP based chromatography.},
  author       = {Spégel, Peter and Schweitz, Leif and Andersson, Lars I. and Nilsson, Staffan},
  issn         = {0009-5893},
  keyword      = {Capillary electrochromatography,Molecularly imprinted polymer,Monolith,Vinylpyridine,Ibuprofen},
  language     = {eng},
  number       = {3-4},
  pages        = {277--285},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Novel Vinylpyridine Based Cationic MIP Monoliths for Enantiomer Separation in CEC},
  url          = {http://dx.doi.org/10.1365/s10337-008-0911-0},
  volume       = {69},
  year         = {2009},
}