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The use of AFLP to find an informative SNP: genetic differences across a migratory divide in willow warblers

Bensch, Staffan LU ; Åkesson, Susanne LU and Irwin, Darren LU (2002) In Molecular Ecology 11(11). p.2359-2366
Abstract
We used the amplified fragment length polymorphism (AFLP) method to obtain genetic markers distinguishing two subspecies of willow warblers Phylloscopus trochilus that have different migratory behaviours but are not differentiated in mitochondrial DNA or at several microsatellite loci. With the inverse-polymerase chain reaction (PCR) approach we converted a dominant AFLP-marker to a codominant single nucleotide polymorphism (SNP). Across Scandinavia we typed 621 birds at the SNP locus AFLP-WW1 and we found a sigmoid change in allele frequencies centred around 62 degrees latitude. North of the latitudinal cline was a west-east cline. Both clines are narrower than one would expect from dispersal distances in willow warblers, which suggests... (More)
We used the amplified fragment length polymorphism (AFLP) method to obtain genetic markers distinguishing two subspecies of willow warblers Phylloscopus trochilus that have different migratory behaviours but are not differentiated in mitochondrial DNA or at several microsatellite loci. With the inverse-polymerase chain reaction (PCR) approach we converted a dominant AFLP-marker to a codominant single nucleotide polymorphism (SNP). Across Scandinavia we typed 621 birds at the SNP locus AFLP-WW1 and we found a sigmoid change in allele frequencies centred around 62 degrees latitude. North of the latitudinal cline was a west-east cline. Both clines are narrower than one would expect from dispersal distances in willow warblers, which suggests that these are maintained by selection. The latitudinal cline at the locus AFLP-WW1 is paralleled by changes in several other traits, all of which might be maintained by a single selective force. The most plausible selection factor that we have identified is selection against hybrids because of inferior migratory behaviour. The selective force maintaining the east-west cline is less obvious. We discuss alternatives to the selection scenario, involving colonization history and asymmetric gene flow. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Ecology
volume
11
issue
11
pages
2359 - 2366
publisher
Wiley-Blackwell
external identifiers
  • wos:000178962500014
  • pmid:12406246
  • scopus:0036846287
ISSN
0962-1083
DOI
10.1046/j.1365-294X.2002.01629.x
language
English
LU publication?
yes
id
b69991a4-b33b-406a-90da-faf477b7c92d (old id 131332)
alternative location
http://dx.doi.org/10.1046/j.1365-294X.2002.01629.x
date added to LUP
2007-06-25 07:48:17
date last changed
2017-09-03 03:50:03
@article{b69991a4-b33b-406a-90da-faf477b7c92d,
  abstract     = {We used the amplified fragment length polymorphism (AFLP) method to obtain genetic markers distinguishing two subspecies of willow warblers Phylloscopus trochilus that have different migratory behaviours but are not differentiated in mitochondrial DNA or at several microsatellite loci. With the inverse-polymerase chain reaction (PCR) approach we converted a dominant AFLP-marker to a codominant single nucleotide polymorphism (SNP). Across Scandinavia we typed 621 birds at the SNP locus AFLP-WW1 and we found a sigmoid change in allele frequencies centred around 62 degrees latitude. North of the latitudinal cline was a west-east cline. Both clines are narrower than one would expect from dispersal distances in willow warblers, which suggests that these are maintained by selection. The latitudinal cline at the locus AFLP-WW1 is paralleled by changes in several other traits, all of which might be maintained by a single selective force. The most plausible selection factor that we have identified is selection against hybrids because of inferior migratory behaviour. The selective force maintaining the east-west cline is less obvious. We discuss alternatives to the selection scenario, involving colonization history and asymmetric gene flow.},
  author       = {Bensch, Staffan and Åkesson, Susanne and Irwin, Darren},
  issn         = {0962-1083},
  language     = {eng},
  number       = {11},
  pages        = {2359--2366},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Ecology},
  title        = {The use of AFLP to find an informative SNP: genetic differences across a migratory divide in willow warblers},
  url          = {http://dx.doi.org/10.1046/j.1365-294X.2002.01629.x},
  volume       = {11},
  year         = {2002},
}