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Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats

Wester, Lena; Koczan, Dirk; Holmberg, Jens LU ; Olofsson, Peter LU ; Thiesen, Hans-Jürgen; Holmdahl, Rikard LU and Ibrahim, Saleh (2003) In Arthritis Research and Therapy 5(6). p.361-372
Abstract
Arthritis susceptibility genes were sought by analysis of differential gene expression between pristane-induced arthritis (PIA)-susceptible DA rats and PIA-resistant E3 rats. Inguinal lymph nodes of nave animals and animals 8 days after pristane injection were analyzed for differential gene expression. mRNA expression was investigated by microarray and real-time PCR, and protein expression was analyzed by flow cytometry or ELISA. Twelve genes were significantly differentially expressed when analyzed by at least two independent methods, and an additional five genes showed a strong a tendency toward differential expression. In nave DA rats IgE, the bone marrow stromal cell antigen 1 (Bst1) and the MHC class II -chain (MhcII) were expressed... (More)
Arthritis susceptibility genes were sought by analysis of differential gene expression between pristane-induced arthritis (PIA)-susceptible DA rats and PIA-resistant E3 rats. Inguinal lymph nodes of nave animals and animals 8 days after pristane injection were analyzed for differential gene expression. mRNA expression was investigated by microarray and real-time PCR, and protein expression was analyzed by flow cytometry or ELISA. Twelve genes were significantly differentially expressed when analyzed by at least two independent methods, and an additional five genes showed a strong a tendency toward differential expression. In nave DA rats IgE, the bone marrow stromal cell antigen 1 (Bst1) and the MHC class II -chain (MhcII) were expressed at a higher level, and the immunoglobulin kappa chain (Ig) was expressed at a lower level. In pristane-treated DA rats the MHC class II -chain, gelatinase B (Mmp9) and the protein tyrosine phosphatase CL100 (Ptpn16) were expressed at a higher level, whereas immunoglobulins, the CD28 molecule (Cd28), the mast cell specific protease 1 (Mcpt1), the carboxylesterase precursor (Ces2), K-cadherin (Cdh6), cyclin G1 (Ccng1), DNA polymerase IV (Primase) and the tumour associated glycoprotein E4 (Tage) were expressed at a lower level. Finally, the differentially expressed mRNA was confirmed with protein expression for some of the genes. In conclusion, the results show that animal models are well suited for reproducible microarray analysis of candidate genes for arthritis. All genes have functions that are potentially important for arthritis, and nine of the genes are located within genomic regions previously associated with autoimmune disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Arthritis Research and Therapy
volume
5
issue
6
pages
361 - 372
publisher
BioMed Central
external identifiers
  • wos:000186151000013
  • pmid:14680511
  • scopus:2442570081
ISSN
1478-6362
DOI
10.1186/ar993
language
English
LU publication?
yes
id
3554553b-8245-40ff-992f-d939ea82814d (old id 131472)
date added to LUP
2007-07-20 08:39:16
date last changed
2017-05-14 03:30:39
@article{3554553b-8245-40ff-992f-d939ea82814d,
  abstract     = {Arthritis susceptibility genes were sought by analysis of differential gene expression between pristane-induced arthritis (PIA)-susceptible DA rats and PIA-resistant E3 rats. Inguinal lymph nodes of nave animals and animals 8 days after pristane injection were analyzed for differential gene expression. mRNA expression was investigated by microarray and real-time PCR, and protein expression was analyzed by flow cytometry or ELISA. Twelve genes were significantly differentially expressed when analyzed by at least two independent methods, and an additional five genes showed a strong a tendency toward differential expression. In nave DA rats IgE, the bone marrow stromal cell antigen 1 (Bst1) and the MHC class II -chain (MhcII) were expressed at a higher level, and the immunoglobulin kappa chain (Ig) was expressed at a lower level. In pristane-treated DA rats the MHC class II -chain, gelatinase B (Mmp9) and the protein tyrosine phosphatase CL100 (Ptpn16) were expressed at a higher level, whereas immunoglobulins, the CD28 molecule (Cd28), the mast cell specific protease 1 (Mcpt1), the carboxylesterase precursor (Ces2), K-cadherin (Cdh6), cyclin G1 (Ccng1), DNA polymerase IV (Primase) and the tumour associated glycoprotein E4 (Tage) were expressed at a lower level. Finally, the differentially expressed mRNA was confirmed with protein expression for some of the genes. In conclusion, the results show that animal models are well suited for reproducible microarray analysis of candidate genes for arthritis. All genes have functions that are potentially important for arthritis, and nine of the genes are located within genomic regions previously associated with autoimmune disease.},
  author       = {Wester, Lena and Koczan, Dirk and Holmberg, Jens and Olofsson, Peter and Thiesen, Hans-Jürgen and Holmdahl, Rikard and Ibrahim, Saleh},
  issn         = {1478-6362},
  language     = {eng},
  number       = {6},
  pages        = {361--372},
  publisher    = {BioMed Central},
  series       = {Arthritis Research and Therapy},
  title        = {Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats},
  url          = {http://dx.doi.org/10.1186/ar993},
  volume       = {5},
  year         = {2003},
}