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Five month persistence of Helicobacter pylori infection in guinea pigs

Sjunnesson, Håkan LU ; Sturegård, Erik LU ; Hynes, Sean LU ; Willén, R; Feinstein, R and Wadström, Torkel LU (2003) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00 111(6). p.634-642
Abstract
Seven Dunkin-Hartley guinea pigs were infected with the Sydney strain of H. pylori (SS1). Gastric histopathology was evaluated and serum antibody response to H. pylori cell-surface proteins was analysed by enzyme immunoassay (EIA) and immunoblot. Tissue and faecal samples from five control animals were analysed for the presence of naturally occurring Helicobacter spp. infection by culture and Helicobacter genus-specific PCR. The H. pylori infection persisted for 5 months, in most animals accompanied by a histologically severe antral gastritis, exhibiting focal degeneration and necrosis of gastric crypt epithelium. Increased numbers of mitotic figures were observed in the gastric epithelium, indicating a regenerative process. Infected... (More)
Seven Dunkin-Hartley guinea pigs were infected with the Sydney strain of H. pylori (SS1). Gastric histopathology was evaluated and serum antibody response to H. pylori cell-surface proteins was analysed by enzyme immunoassay (EIA) and immunoblot. Tissue and faecal samples from five control animals were analysed for the presence of naturally occurring Helicobacter spp. infection by culture and Helicobacter genus-specific PCR. The H. pylori infection persisted for 5 months, in most animals accompanied by a histologically severe antral gastritis, exhibiting focal degeneration and necrosis of gastric crypt epithelium. Increased numbers of mitotic figures were observed in the gastric epithelium, indicating a regenerative process. Infected animals displayed specific antibodies towards H. pylori cell-surface proteins in immunoblot, whereas EIA was of dubious value creating false-positive results. Serum complement C3 and cholesterol levels appeared to be elevated in infected animals. Helicobacter spp. infection was not detected in the control animals. The persistent infection, accompanied by severe gastritis and a prominent serum antibody response, and the apparent absence of a natural Helicobacter spp. infection makes the guinea pig model useful in H. pylori research. (Less)
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publication status
published
subject
in
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00
volume
111
issue
6
pages
634 - 642
publisher
John Wiley & Sons
external identifiers
  • wos:000185478100006
  • pmid:12969019
  • scopus:0141519218
ISSN
1600-0463
DOI
language
English
LU publication?
yes
id
76d6b3f4-ae77-474a-a323-6d6d99bcf442 (old id 132285)
date added to LUP
2007-07-10 14:19:36
date last changed
2018-05-29 10:22:21
@article{76d6b3f4-ae77-474a-a323-6d6d99bcf442,
  abstract     = {Seven Dunkin-Hartley guinea pigs were infected with the Sydney strain of H. pylori (SS1). Gastric histopathology was evaluated and serum antibody response to H. pylori cell-surface proteins was analysed by enzyme immunoassay (EIA) and immunoblot. Tissue and faecal samples from five control animals were analysed for the presence of naturally occurring Helicobacter spp. infection by culture and Helicobacter genus-specific PCR. The H. pylori infection persisted for 5 months, in most animals accompanied by a histologically severe antral gastritis, exhibiting focal degeneration and necrosis of gastric crypt epithelium. Increased numbers of mitotic figures were observed in the gastric epithelium, indicating a regenerative process. Infected animals displayed specific antibodies towards H. pylori cell-surface proteins in immunoblot, whereas EIA was of dubious value creating false-positive results. Serum complement C3 and cholesterol levels appeared to be elevated in infected animals. Helicobacter spp. infection was not detected in the control animals. The persistent infection, accompanied by severe gastritis and a prominent serum antibody response, and the apparent absence of a natural Helicobacter spp. infection makes the guinea pig model useful in H. pylori research.},
  author       = {Sjunnesson, Håkan and Sturegård, Erik and Hynes, Sean and Willén, R and Feinstein, R and Wadström, Torkel},
  issn         = {1600-0463},
  language     = {eng},
  number       = {6},
  pages        = {634--642},
  publisher    = {John Wiley & Sons},
  series       = { APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00},
  title        = {Five month persistence of Helicobacter pylori infection in guinea pigs},
  url          = {http://dx.doi.org/},
  volume       = {111},
  year         = {2003},
}