Advanced

Serum antibodies to Helicobacter hepaticus and Helicobacter pylori in patients with chronic liver disease

Nilsson, Ingrid LU ; Lindgren, S; Eriksson, S and Wadström, Torkel LU (2000) In Gut 46(3). p.410-414
Abstract
BACKGROUND: Bile tolerant helicobacter species such as H hepaticus and H bilis have frequently been reported to cause hepatitis in mice and other rodents.

AIMS: To investigate the possible pathogenic role of these and other helicobacter species in chronic liver disease in humans.

METHODS: Serum samples from 144 patients with various chronic liver diseases, 30 patients with primary sclerosing cholangitis (PSC), and 48 healthy blood donors were analysed for antibodies against H hepaticus murine strain CCUG 33637 and H pylori strain CCUG 17874. Cell surface proteins of H hepaticus were extracted by acid glycine buffer and used in an enzyme immunoassay (EIA) and immunoblot (IB).

RESULTS: 56 of 144 (39%) patients with... (More)
BACKGROUND: Bile tolerant helicobacter species such as H hepaticus and H bilis have frequently been reported to cause hepatitis in mice and other rodents.

AIMS: To investigate the possible pathogenic role of these and other helicobacter species in chronic liver disease in humans.

METHODS: Serum samples from 144 patients with various chronic liver diseases, 30 patients with primary sclerosing cholangitis (PSC), and 48 healthy blood donors were analysed for antibodies against H hepaticus murine strain CCUG 33637 and H pylori strain CCUG 17874. Cell surface proteins of H hepaticus were extracted by acid glycine buffer and used in an enzyme immunoassay (EIA) and immunoblot (IB).

RESULTS: 56 of 144 (39%) patients with chronic liver diseases and six of 30 (20%) with PSC showed increased antibody concentrations in the H hepaticus EIA; in the H pylori EIA the numbers were 58% and 13% respectively. Compared with the healthy blood donors the antibody reactivity against the two helicobacter species was not increased (46% and 48% respectively). Patient serum samples retested by the H hepaticus EIA after absorption with sonicated H pylori cells remained positive in 12 of 37 (33%) serum samples. Distinct antibody reactivity to 55-65 kDa proteins was observed by H hepaticus IB, after the absorption step, and was considered specific for H hepaticus. These 12 serum samples were from patients with chronic alcoholic liver disease.

CONCLUSIONS: Antibodies to H hepaticus, often cross reacting with H pylori, occur frequently in patients with chronic liver diseases, with no clear cut relation to specific diagnostic groups. The pathogenic significance of these findings is not known. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Gut
volume
46
issue
3
pages
410 - 414
publisher
BMJ Publishing Group
external identifiers
  • wos:000085489600023
  • scopus:0034070869
ISSN
1468-3288
language
English
LU publication?
yes
id
11c4b969-7905-4c64-9807-310a487b4c7b (old id 132394)
alternative location
http://gut.bmjjournals.com/cgi/content/abstract/46/3/410
date added to LUP
2007-07-10 08:55:16
date last changed
2017-07-02 04:27:06
@article{11c4b969-7905-4c64-9807-310a487b4c7b,
  abstract     = {BACKGROUND: Bile tolerant helicobacter species such as H hepaticus and H bilis have frequently been reported to cause hepatitis in mice and other rodents.<br/><br>
AIMS: To investigate the possible pathogenic role of these and other helicobacter species in chronic liver disease in humans.<br/><br>
METHODS: Serum samples from 144 patients with various chronic liver diseases, 30 patients with primary sclerosing cholangitis (PSC), and 48 healthy blood donors were analysed for antibodies against H hepaticus murine strain CCUG 33637 and H pylori strain CCUG 17874. Cell surface proteins of H hepaticus were extracted by acid glycine buffer and used in an enzyme immunoassay (EIA) and immunoblot (IB).<br/><br>
RESULTS: 56 of 144 (39%) patients with chronic liver diseases and six of 30 (20%) with PSC showed increased antibody concentrations in the H hepaticus EIA; in the H pylori EIA the numbers were 58% and 13% respectively. Compared with the healthy blood donors the antibody reactivity against the two helicobacter species was not increased (46% and 48% respectively). Patient serum samples retested by the H hepaticus EIA after absorption with sonicated H pylori cells remained positive in 12 of 37 (33%) serum samples. Distinct antibody reactivity to 55-65 kDa proteins was observed by H hepaticus IB, after the absorption step, and was considered specific for H hepaticus. These 12 serum samples were from patients with chronic alcoholic liver disease.<br/><br>
CONCLUSIONS: Antibodies to H hepaticus, often cross reacting with H pylori, occur frequently in patients with chronic liver diseases, with no clear cut relation to specific diagnostic groups. The pathogenic significance of these findings is not known.},
  author       = {Nilsson, Ingrid and Lindgren, S and Eriksson, S and Wadström, Torkel},
  issn         = {1468-3288},
  language     = {eng},
  number       = {3},
  pages        = {410--414},
  publisher    = {BMJ Publishing Group},
  series       = {Gut},
  title        = {Serum antibodies to Helicobacter hepaticus and Helicobacter pylori in patients with chronic liver disease},
  volume       = {46},
  year         = {2000},
}