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Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.

Wiklund, M; Spégel, Peter LU ; Nilsson, Staffan LU and Hertz, H M (2003) In Ultrasonics 41(4). p.329-333
Abstract
We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical... (More)
We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ultrasonic trapping, Capillary electrophoresis, Particle separation, Concentration limit of detection
in
Ultrasonics
volume
41
issue
4
pages
329 - 333
publisher
Elsevier
external identifiers
  • wos:000183679100015
  • scopus:0038182656
ISSN
0041-624X
DOI
10.1016/S0041-624X(02)00460-2
language
English
LU publication?
yes
id
27369816-d6b4-4e79-8f22-c33ad4503d35 (old id 132765)
date added to LUP
2007-06-27 16:39:15
date last changed
2017-07-23 04:45:42
@article{27369816-d6b4-4e79-8f22-c33ad4503d35,
  abstract     = {We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement.},
  author       = {Wiklund, M and Spégel, Peter and Nilsson, Staffan and Hertz, H M},
  issn         = {0041-624X},
  keyword      = {Ultrasonic trapping,Capillary electrophoresis,Particle separation,Concentration limit of detection},
  language     = {eng},
  number       = {4},
  pages        = {329--333},
  publisher    = {Elsevier},
  series       = {Ultrasonics},
  title        = {Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.},
  url          = {http://dx.doi.org/10.1016/S0041-624X(02)00460-2},
  volume       = {41},
  year         = {2003},
}