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Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus.

Träff, Karin LU ; Lönn, Anna LU ; Otero Cordera, R R; van Zyl,, W H and Hahn-Hägerdal, Bärbel LU (2003) In Enzyme and Microbial Technology 32(5). p.567-573
Abstract
Three xylose isomerase enzymes (XI) [Eur. J. Biochem. 269 (2002) 157], encoded by mutated xylA genes from Thermus thermophilus, were produced at two different levels in Saccharomyces cerevisiae; xylA genes were chromosomally integrated and expressed from multicopy plasmids, respectively. An extra copy of the endogenous xylulokinase gene (XKS1) was chromosomally integrated and the aldose reductase (AR) GRE3 gene was deleted. Ethanol was formed from xylose only when xylA was expressed from multicopy plasmids and when the specific XI activity was higher than 30 mU/mg protein. Deletion of the GRE3 gene was crucial for ethanol formation, possibly because reduced xylitol formation caused less inhibition of XI.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Xylose fermentation, Xylose, Xylose isomerase, Xylulokinase, Saccharomyces cerevisiae, GRE3
in
Enzyme and Microbial Technology
volume
32
issue
5
pages
567 - 573
publisher
Elsevier
external identifiers
  • wos:000181908500010
  • scopus:0242669383
ISSN
0141-0229
DOI
10.1016/S0141-0229(03)00024-3
language
English
LU publication?
yes
id
5dc0b9e7-4d07-4ad8-9b77-34b183c67d8a (old id 132840)
date added to LUP
2007-06-28 14:28:57
date last changed
2018-01-07 05:32:36
@article{5dc0b9e7-4d07-4ad8-9b77-34b183c67d8a,
  abstract     = {Three xylose isomerase enzymes (XI) [Eur. J. Biochem. 269 (2002) 157], encoded by mutated xylA genes from Thermus thermophilus, were produced at two different levels in Saccharomyces cerevisiae; xylA genes were chromosomally integrated and expressed from multicopy plasmids, respectively. An extra copy of the endogenous xylulokinase gene (XKS1) was chromosomally integrated and the aldose reductase (AR) GRE3 gene was deleted. Ethanol was formed from xylose only when xylA was expressed from multicopy plasmids and when the specific XI activity was higher than 30 mU/mg protein. Deletion of the GRE3 gene was crucial for ethanol formation, possibly because reduced xylitol formation caused less inhibition of XI.},
  author       = {Träff, Karin and Lönn, Anna and Otero Cordera, R R and van Zyl,, W H and Hahn-Hägerdal, Bärbel},
  issn         = {0141-0229},
  keyword      = {Xylose fermentation,Xylose,Xylose isomerase,Xylulokinase,Saccharomyces cerevisiae,GRE3},
  language     = {eng},
  number       = {5},
  pages        = {567--573},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus.},
  url          = {http://dx.doi.org/10.1016/S0141-0229(03)00024-3},
  volume       = {32},
  year         = {2003},
}