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Function of Innate Immune Cells in Breast Cancer

Gunnarsdóttir, Fríða Björk LU (2021) In Lund University, Faculty of Medicine Doctoral Dissertation Series
Abstract
Tumor associated macrophages (TAMs) are key cells in creating an immunosuppressive tumor microenvironment (TME). In general, presence of TAMs is associated with worse outcome in cancer patients. Macrophages with anti-tumor effect can be found in the TME but are usually in minority. This thesis focuses on the role of innate immune cells, and especially macrophages, in breast cancer.

In the first project we showed that pro-inflammatory macrophages downregulate estrogen receptor alpha (ERα) on breast cancer cells. We unveiled the molecular mechanism behind this, showing that TNF-α derived from macrophages inactivates transcription factor FOXO3a. Moreover, presence of TAMs in breast cancer tumors associated with ER negativity and... (More)
Tumor associated macrophages (TAMs) are key cells in creating an immunosuppressive tumor microenvironment (TME). In general, presence of TAMs is associated with worse outcome in cancer patients. Macrophages with anti-tumor effect can be found in the TME but are usually in minority. This thesis focuses on the role of innate immune cells, and especially macrophages, in breast cancer.

In the first project we showed that pro-inflammatory macrophages downregulate estrogen receptor alpha (ERα) on breast cancer cells. We unveiled the molecular mechanism behind this, showing that TNF-α derived from macrophages inactivates transcription factor FOXO3a. Moreover, presence of TAMs in breast cancer tumors associated with ER negativity and worse prognosis in ERα+ patients.

In projects two and three we shifted our focus towards CD169+ macrophages in lymph nodes (LN) and primary tumors (PT) of breast cancer patients. In project II we showed that presence of CD169+ macrophages in metastatic LN correlated with better prognosis, while presence of CD169+ macrophages in PT did not. Association with PD-L1 expression was found in both locations.

In project III we saw that CD169+ TAMs are most likely monocyte derived in a type I IFN environment and display a unique pro-inflammatory phenotype and cytokine profile, but with immunosuppressive function. In a patient cohort they were associated with tertiary lymphoid structures and regulatory T cells, and therefore with worse prognosis.
In conclusion, TAMs represent a broad spectrum of macrophages with unique origin, phenotype, and function. In this thesis we have added to the growing knowledge of these cells and their role in breast cancer. Not only does the type of cancer matter for their function, but further their location and surrounding environment within breast cancer.
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author
supervisor
opponent
  • Professor Dabrosin, Charlotta, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden.
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Tumor associated macrophages, Breast cancer, Estrogen receptor alpha, FOXO3a, CD169, PD-L1, metastatic lymph node
in
Lund University, Faculty of Medicine Doctoral Dissertation Series
issue
2021:140
pages
77 pages
publisher
Lund University, Faculty of Medicine
defense location
Agardh föreläsningssal, CRC, Jan Waldenströms gata 35, Skånes Universitetssjukhus i Malmö. Join by Zoom: https://lu-se.zoom.us/j/61297955930
defense date
2021-12-03 09:15:00
ISSN
1652-8220
ISBN
978-91-8021-147-5
language
English
LU publication?
yes
id
13364104-e008-4d32-b5d5-c115ac2032d4
date added to LUP
2021-11-12 21:38:36
date last changed
2022-08-05 08:34:10
@phdthesis{13364104-e008-4d32-b5d5-c115ac2032d4,
  abstract     = {{Tumor associated macrophages (TAMs) are key cells in creating an immunosuppressive tumor microenvironment (TME). In general, presence of TAMs is associated with worse outcome in cancer patients. Macrophages with anti-tumor effect can be found in the TME but are usually in minority. This thesis focuses on the role of innate immune cells, and especially macrophages, in breast cancer. <br/><br/>In the first project we showed that pro-inflammatory macrophages downregulate estrogen receptor alpha (ERα) on breast cancer cells. We unveiled the molecular mechanism behind this, showing that TNF-α derived from macrophages inactivates transcription factor FOXO3a. Moreover, presence of TAMs in breast cancer tumors associated with ER negativity and worse prognosis in ERα<sup>+</sup> patients. <br/><br/>In projects two and three we shifted our focus towards CD169<sup>+</sup> macrophages in lymph nodes (LN) and primary tumors (PT) of breast cancer patients. In project II we showed that presence of CD169<sup>+</sup> macrophages in metastatic LN correlated with better prognosis, while presence of CD169<sup>+</sup> macrophages in PT did not. Association with PD-L1 expression was found in both locations. <br/><br/>In project III we saw that CD169<sup>+</sup> TAMs are most likely monocyte derived in a type I IFN environment and display a unique pro-inflammatory phenotype and cytokine profile, but with immunosuppressive function. In a patient cohort they were associated with tertiary lymphoid structures and regulatory T cells, and therefore with worse prognosis.<br/>In conclusion, TAMs represent a broad spectrum of macrophages with unique origin, phenotype, and function. In this thesis we have added to the growing knowledge of these cells and their role in breast cancer. Not only does the type of cancer matter for their function, but further their location and surrounding environment within breast cancer.<br/>}},
  author       = {{Gunnarsdóttir, Fríða Björk}},
  isbn         = {{978-91-8021-147-5}},
  issn         = {{1652-8220}},
  keywords     = {{Tumor associated macrophages; Breast cancer; Estrogen receptor alpha; FOXO3a; CD169; PD-L1; metastatic lymph node}},
  language     = {{eng}},
  number       = {{2021:140}},
  publisher    = {{Lund University, Faculty of Medicine}},
  school       = {{Lund University}},
  series       = {{Lund University, Faculty of Medicine Doctoral Dissertation Series}},
  title        = {{Function of Innate Immune Cells in Breast Cancer}},
  url          = {{https://lup.lub.lu.se/search/files/109642259/FridaBjorkGunnarsdottirThesis.pdf}},
  year         = {{2021}},
}