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DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo.

Padash, Mojgan LU ; Rogers, Stephen and Häcker, Udo LU (2005) In Journal of Cell Biology 168(4). p.575-585
Abstract
Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose... (More)
Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Cell Biology
volume
168
issue
4
pages
575 - 585
publisher
Rockefeller University Press
external identifiers
  • wos:000227101100024
  • pmid:15699213
  • scopus:13944278366
ISSN
0021-9525
DOI
10.1083/jcb.200407124
language
English
LU publication?
yes
id
f4740509-5ccd-4360-a4b0-46ac4590311a (old id 133858)
date added to LUP
2007-07-18 14:52:24
date last changed
2017-10-29 03:44:41
@article{f4740509-5ccd-4360-a4b0-46ac4590311a,
  abstract     = {Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors.},
  author       = {Padash, Mojgan and Rogers, Stephen and Häcker, Udo},
  issn         = {0021-9525},
  language     = {eng},
  number       = {4},
  pages        = {575--585},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Cell Biology},
  title        = {DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo.},
  url          = {http://dx.doi.org/10.1083/jcb.200407124},
  volume       = {168},
  year         = {2005},
}