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Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana

Li, S S; Wang, Y and Björn, Lars Olof LU (2004) In Journal of Environmental Sciences(China) 16(1). p.173-176
Abstract
DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 64PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24degreesC was much faster than that at 0degreesC and 12degreesC with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at... (More)
DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 64PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24degreesC was much faster than that at 0degreesC and 12degreesC with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12degreesC was very slow as compared with that at 24degreesC and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0degreesC. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Environmental Sciences(China)
volume
16
issue
1
pages
173 - 176
publisher
Science China Press
external identifiers
  • pmid:14971476
  • wos:000188701000036
  • scopus:0842334488
ISSN
1001-0742
project
Photobiology
language
English
LU publication?
yes
id
1a0bcabe-5ce5-46d7-a0d9-b7077b622946 (old id 134346)
alternative location
http://www.wanfangdata.com.cn/qikan/periodical.Articles/jes-e/jes-2004/0401/040136.htm
date added to LUP
2007-07-30 09:34:24
date last changed
2017-01-22 03:27:37
@article{1a0bcabe-5ce5-46d7-a0d9-b7077b622946,
  abstract     = {DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 64PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24degreesC was much faster than that at 0degreesC and 12degreesC with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12degreesC was very slow as compared with that at 24degreesC and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0degreesC. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.},
  author       = {Li, S S and Wang, Y and Björn, Lars Olof},
  issn         = {1001-0742},
  language     = {eng},
  number       = {1},
  pages        = {173--176},
  publisher    = {Science China Press},
  series       = {Journal of Environmental Sciences(China)},
  title        = {Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana},
  volume       = {16},
  year         = {2004},
}