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Light-induced linear dichroism in photoreversibly photochromic sensor pigments 3. Chromophore rotation estimated by polarized-light reversal of dichroism

Sundqvist, C and Björn, Lars Olof LU (1983) In Physiologia Plantarum 59(2). p.263-269
Abstract
Phytochrome from oats (Avena sativa L. cv. Sol II), partially purified on brushite, was immobilized on Sepharose beads to which antiphytochrome immunoglobulin had been covalently linked. The immobilized phytochrome was first brought to the Pr form with unpolarized far-red light. The change in linear dichroism at 660 nm induced by plane polarized red light, and its reversal by plane polarized far-red light were then studied using a dual-wavelength spectrophotometer equipped with polarizing filters. The far-red light was most effective in reversing red-induced dichroism when the angle between the planes of polarization of red and far-red light was approximately 23.degree.. From this it was computed that the long-wavelength transition moment... (More)
Phytochrome from oats (Avena sativa L. cv. Sol II), partially purified on brushite, was immobilized on Sepharose beads to which antiphytochrome immunoglobulin had been covalently linked. The immobilized phytochrome was first brought to the Pr form with unpolarized far-red light. The change in linear dichroism at 660 nm induced by plane polarized red light, and its reversal by plane polarized far-red light were then studied using a dual-wavelength spectrophotometer equipped with polarizing filters. The far-red light was most effective in reversing red-induced dichroism when the angle between the planes of polarization of red and far-red light was approximately 23.degree.. From this it was computed that the long-wavelength transition moment of phytochrome rotates about 29.degree. (or 180-29.degree.) with respect to the protein during conversion from Pr to Pfr. The reverse experiment, using unpolarized red light followed first by polarized far-red light and then polarized red light, with dichroism monitored at 730 nm, also gives most effective reversal for an angle of about 23.degree. between polarization planes, but this corresponds to a transition moment rotation of about 36.degree. (or 180.degree.-36.degree.). The present method is more straightforward but less accurate and confirms an earlier conclusion that the rotation angle is close to 32.degree. (or 180.degree.-32.degree.) in contrast to the "in vivo" value of 90.degree. found by several workers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physiologia Plantarum
volume
59
issue
2
pages
263 - 269
publisher
Wiley Online Library
external identifiers
  • scopus:84989759814
ISSN
0031-9317
DOI
10.1111/j.1399-3054.1983.tb00769.x
language
English
LU publication?
yes
id
9ec760f3-38b1-4969-8da2-2ae5c60de4c4 (old id 134614)
date added to LUP
2007-07-30 11:14:04
date last changed
2017-09-17 07:12:34
@article{9ec760f3-38b1-4969-8da2-2ae5c60de4c4,
  abstract     = {Phytochrome from oats (Avena sativa L. cv. Sol II), partially purified on brushite, was immobilized on Sepharose beads to which antiphytochrome immunoglobulin had been covalently linked. The immobilized phytochrome was first brought to the Pr form with unpolarized far-red light. The change in linear dichroism at 660 nm induced by plane polarized red light, and its reversal by plane polarized far-red light were then studied using a dual-wavelength spectrophotometer equipped with polarizing filters. The far-red light was most effective in reversing red-induced dichroism when the angle between the planes of polarization of red and far-red light was approximately 23.degree.. From this it was computed that the long-wavelength transition moment of phytochrome rotates about 29.degree. (or 180-29.degree.) with respect to the protein during conversion from Pr to Pfr. The reverse experiment, using unpolarized red light followed first by polarized far-red light and then polarized red light, with dichroism monitored at 730 nm, also gives most effective reversal for an angle of about 23.degree. between polarization planes, but this corresponds to a transition moment rotation of about 36.degree. (or 180.degree.-36.degree.). The present method is more straightforward but less accurate and confirms an earlier conclusion that the rotation angle is close to 32.degree. (or 180.degree.-32.degree.) in contrast to the "in vivo" value of 90.degree. found by several workers.},
  author       = {Sundqvist, C and Björn, Lars Olof},
  issn         = {0031-9317},
  language     = {eng},
  number       = {2},
  pages        = {263--269},
  publisher    = {Wiley Online Library},
  series       = {Physiologia Plantarum},
  title        = {Light-induced linear dichroism in photoreversibly photochromic sensor pigments 3. Chromophore rotation estimated by polarized-light reversal of dichroism},
  url          = {http://dx.doi.org/10.1111/j.1399-3054.1983.tb00769.x},
  volume       = {59},
  year         = {1983},
}